Effects Of Glucose On The Expressions Of OPG And TRAIL In MG63 Cells

Diabetic complications such as osteoporosis have become one of major causes of death, disability and increment of medicare. So, it is important to explore the pathogenesis of diabetic osteoporosis. Recently, novel members of Tumor Necrosis Factor family such as osteoprotegerin(OPG), osteoprotegerin ligand (OPGL), and tumor necrosis factor related apoptosis inducing ligand (TRAIL), macrophage colony stimulating factor (M-CSF), transforming growth factor-β (TGF-β), etc. are reported being involved in regulation of osteoclast' s formation and differentiation. However their roles in diabetic osteoporosis are not clear. The effects of glucose in different concentration on the mRNA expressions of OPG, OPGL, TRAIL in MG63 cells were observed to explore the pathogenesis of diabetic osteoporosis.METHODS: Osteoblast-like MG63 cells were divided into 4 groups based on the concentrations of glucose in MEM culture medium:...