The Effect Of Xing Nao Jing Act On Vascular Endothelial Cell’s Survial Activity After OGD Injury And Inhibiting The Inflammatory Reaction Mechanism In Vitro Study

Objective: To research the protective effect of Xing nao jing whether have thefunction of vascular endothelial cell’s survial activity after OGD damage andinhibit the inflammatory reaction of its mechanism.Method: Firstly based the OGD injury model of vascular endothelial cell withNa2S2O4and sugar free MEM medium. By using CCK8assay To clear whetherXing naojing injection act on cellular toxicity or not,and then observe theeffects of vascular endothelial cell activity by Na2S2O4in differentconcentrations act on vascular endothelial cells after3h,then change in normalculture medium for another24h and48h. observe the vascular endothelial cellsexpression of NF-kb, TGF-βafter OGD damage by immunefluorescencemethod(IFA); Determination of the concentration of IL-6in supernatant ofendothelial cells by ELISA method.Results:1、compared with thedifference between xing nao jing control groupand the blank control group. Measured by the CCK8method of OD value in24hXing nao jing control group and calculate the cell viability of vascularendothelial cells which were compared with the blank control group in1:25,1:50,1:100three groups of Xing nao jing concentration were1.103948±0.075501:,0.984523±0.049329,0.970015±0.128293; the vascular endothelialcell viability in48h Xing nao jing control group were:0.932485±0.01159activity,0.932485±0.025775,0.932485±0.012166. No significant differenceof income data,,so we can see that xing nao jing for HMEC without cytotoxicity.Na2S2O4model group compared to blank control group, HMEC activity ofNa2S2O4model group decreased significantly, and with the higher concentration of Na2S2O4(5mmol、10mmol、20mmol) the percentage of endothelial cell’sactivity:66.617%、67.164%、44.129%and73.210%、71.296%、48.951%; Thexing nao jing experimental group compared with the Na2S2O4model group,the percentage of endothelial cell’s activity:73.582%、63.184%、43.582%and82.037%、82.099%、43.559%. So the effect of Xing nao jing group act in48hvisible batter than the action in the24h group, while5umol,10umol Na2S2O4model group after Xingnaojing effect is better than the effect of group20umol,namely the hypoxic injury is heavier, the recovery of HMEC activity would bemore difficult.2、The Na2S2O4model group compared with blank control group, NF-κB,TGF-β fiuorescence staining was enhanced, Obviously the immune fluorescencevalue of NF-κB and TGF-β in Na2S2O4model group were61.642±10.81and53.994±4.443, significantly higher than that of the control group were21.394±3.454and15.694±2.390by immune fluorescence value. At the same time, NF-κB nuclear transfer increased significantl under the confocal microscope. Butcompared with the xing nao jing experimental group and Na2S2O4model group,NF-κB, TGF-β fiuorescence staining were weakened obvivously,and it’simmune fluorescence value were31.864±2.967and31.580±5.158,and NF-κBnuclear transfer is just a scattered phenomenon.3、The Na2S2O4model group (55.702±1.762)compared with blank control group（16.692±0.978), the concentration of IL-6values were significantly higher,itmeans the injury of HMEC increased the expression of IL-6;however theconcentrate numerical value of IL-6in Xing nao jing experimental group was30.778±5.803, compared with the Na2S2O4model group, IL-6concentrationdecreased significantly.Conclusion:1, Xing nao jing is safe and reliable, has a protective effect on theproliferation activity of vascular endothelial cell.2, Xingnaojing can reduce the High expression of NF-κB, TGF-β, and therefore reduce the risk of thrombosisand limit vascular pathological changes and development.3, Xingnaojinginjection can inhibit the excessive activation of IL-6, has definiteanti-inflammatory effect.