| Background:Colorectal Carcinomas was one of the common human gastrointestinal malignancy.Its occurrence and development was a multi-step process, and involved multiple factors.In recent years, the incidence of colorectal Carcinomas is gradually increased, itsprevention, diagnosis and treatment has become the research focus of scholars. CD24was the ligand of P-selectin. It involved in the adhesion and matrix between cells.Studies have shown that CD24and PDGF-D, involved in the development and transferprocess of multiple tumor cells. Studies have shown that esophageal cancer invasion andlymph node metastasis were related to the overexpression of CD24. It can be used as aprognostic indicator in patients with esophageal cancer. Animal experiments showed thatCD24can regulate the proliferation and differentiation of cells, and can promote tumorcell growth and proliferation. Platelet-derived growth factor D（PDGF-D）came frommesenchymal cells, such as mononuclear macrophage and fibroblast cells. It participatedin the roles of cell division and immunity induction in vivo. PDGF-D can promote theproliferation of hepatoma cell lines and inhibit their apoptosis. The interaction betweenPDGF-D and PDGFR β could activate its downstream signaling pathways, leading totumor development. In the occurrence of liver cancer and metastasis, PDGF-D andvascular endothelial growth factor（VEGF）play a synergistic role. It may become aprediction indicators of liver cancer prognosis and to guide targeted therapy ofhepatocellular carcinoma. PDGF-D induced the activation of Notch-1and NF-κB, thuspromoting the invasion and metastasis of pancreatic cancer. Experimental data showedthat the down-regulation of PDGF-D could inactivate Notch-1and NF-κB, andlowered their target gene（MMP-9and VEGF）. It could inhibite the growth and vasculargeneration of pancreatic cancer cells to play the anti-tumor effect. However, there are rarereports of CD24and PDGF-D in Colorectal Carcinomas. In order to investigate the roles of CD24and PDGF-D in the occurrence and development of Colorectal Carcinomas. Inthis study, reverse transcriptase polymerase chain reaction（RT-PCR）was used to detectthe expression level of CD24mRNA and PDGF-D mRNA in Colorectal Carcinomas andcorresponding normal tissues. To analyze the relationship between them and clinicalpathology of Colorectal Carcinomas.Objective:To detect the expression level of CD24mRNA and PDGF-D mRNA in ColorectalCarcinomas, to explore their signifiation in the development and progression of ColorectalCarcinomas, and to analyze the relationship between them and clinical pathology ofColorectal Carcinomas. To provide new indicator for the early diagnosis and prognostic ofColorectal Carcinomas, and to provide new ideas for the gene-targeted therapy ofColorectal Carcinomas.Methods:It was collected that46cases of Colorectal Carcinomas and corresponding normaltissues. The specimens were placed in labeled vials in liquid nitrogen spare. Allspecimens were confirmed by two senior physician of Pathology. Reverse transcriptasepolymerase chain reaction（RT-PCR）was used to detect the expression level of CD24mRNA and PDGF-D mRNA in all cases. SPSS17.0statistical software was used toanalyze the statistical significance of CD24mRNA’s and PDGF-D mRNA’s expression inColorectal Carcinomas, and was used to analyze their correlation with clinicopathologicalfactors of Colorectal Carcinomas.Results:1. The expression of CD24mRNA and PDGF-D mRNA were higher in ColorectalCarcinomas than that in normal tissues（0.8322±0.0856:0.2065±0.0871,0.8482±0.0692:0.2533±0.0716）, P <0.05. The difference was statistically significant.2. The expression of CD24mRNA was correlated with tumor invasion（P <0.05）.The difference was statistically significant, and was not correlated with age, degree ofdifferentiation, Duke ’s stages and diameter of tumor（P>0.05）. The difference was notstatistically significant. The expression of PDGF-D mRNA was correlated with age,degree of differentiation, Duke ’s stages（P <0.05）. The difference was statisticallysignificant, and was not corrdlated with tumor invasion and diameter of tumor (P>0.05).The difference was not statistically significant.3. Correlation analysis showed that there was a positive correlation between CD24 mRNA and PDGF-D mRNA in Colorectal Carcinomas（rs=0.383, P=0.004<0.05）.The difference was statistically significant.Conclusion:1. In Colorectal Carcinomas, the expression of CD24mRNA and PDGF-D mRNAwere higher than that in corresponding normal tissues, indicating that both were highlyexpressed in Colorectal Carcinomas and played important roles in the development andprogression of Colorectal Carcinomas.2. The expression of CD24mRNA and tumor invasion of Colorectal Carcinomas waspositively correlated, suggesting that CD24may be involved in the process of invasionand metastasis.The expression of PDGF-D mRNA was positively correlated with with age, degreeof malignancy, Duke ’s stages. PDGF-D may be involved in the invasion and metastasisof Colorectal Carcinomas, and may be related to the prognosis of patients with ColorectalCarcinomas.3. Correlation analysis showed that CD24mRNA and PDGF-D mRNA expressionin Colorectal Carcinomas tissues were positively correlated, suggesting that they may besynergistic to participate in the development and progression of Colorectal Carcinomas.4. High expression of CD24mRNA and PDGF-D mRNA were closely correlatedwith the occurrence and development of Colorectal Carcinomas. They might serve as anew indicator for early diagnosis and perdicting the prognosis of Colorectal Carcinomas.They might provide new ideas for Colorectal Carcinomas’ targeted therapy. |
