| Many studies had shown that the host genotype, gender, age, physical and psychological conditions and other factors had a significant impact on the composition of the human fecal microflora. However, There was relatively less monographic study about lifestyle factors affecting the intestinal microbial. Along with the speeding up of the urbanization, the lifestyle differences in Mongolian population increased constantly. The herders still living in the pastoral followed the traditional way of life for thousands of years. They had a nomadic life and ate beef, mutton and dairy products primarily; While the Mongolian migrating to the cities enjoyed the advanced achievements of modern civilization, and their diet had also undergone a decided change. Their diet included much more vegetables and fruits in proportion to less dairy and meat. Therefore, the choice of two above the crowd as the object of study was to understand the impact of lifestyle on the composition of the fecal microbiota better.The study choose Mongolian healthy youth (Age:19-35years, BMI:18.5-24.0kg/m2) living in Xilinhot and Hohhot as the research object, and analyzed the diversity and quantity of intestinal flora by PCR-DGGE and q-PCR. The study found that the largest and second-largest dominant bacterium in intestinal flora of both crowd were Firmicutes and Bacteroidetes. Ruminococcus, Faecalibacterium prausnitzii and gamma-proteobacteria outlined the most advantage of the human intestinal bacteria. Yet, studies also found that the amounts of the Atopobium cluster, Bacteroides group, Desulfovibrio genus, Faecalibacterium prausnitzii, Lactobacillus and Bifidobacterium in the gut of the youth living in the pastoral area were higher than in the urban environments, and the interclass difference of Bifidobacterium patency (p<0.01). In contrast, the content of Clostridium in the pastoral gut was significantly lower than that in the urban (p<0.001). Qualitative analysis for common strain of Lactobacillus and Bifidobacterium revealed that L. Casei existed in all the individuals of the two groups. the exist ratio of L. plantarum in the population of pastoral areas is slightly lower than the urban population, but the detection ate of L. Brevis, L. Acidophilus, L. helveticus and L. Fermentum in the intestinal flora of pastoral group were higher than that in the urban group. Apart from B. Angulatum, the occurrence rate of other familiar Bifidobacterium such as B. infantis, B. longum, B. Breve and B. Adolescentis in the pastoral youth were higher than in the urban, moreover, there was a large difference about the detection rate of B. Infantis and B. Adolescentis between two groups. Thus it could be seen that there was a big difference between two kinds of Mongolian who had different lifestyle about the composition of the human fecal microflora.Determination of organic acids in the feces showed the content of total bile acid (TBA) in the pastoral population was slightly lower than in the urban population, but acetic acid and propionic acid in former group were higher than in later group. The study also found that the content of Clostridium and Carbohydrate intake was positively correlated (p<0.01, r=0.50), negative correlation between Clostridium and fat intake also were founded (p<0.01,r=-0.60). Hence, one conclusion was drawn in this paper that different lifestyle in diet, especially intake of fermented dairy products might the main reason for different fecal microbiota between pastoral Mongolian and urban Mongolian. The project aimed to provide certain reference for the basic research about the Mongolian fecal microflora and also to explore the shape of the diet and other external factors on the fecal microbiota. |
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