The Analysis Of Genetic Diversity Of Amomum Tsaoko (Zingiberaceae)and The Molecular Identification Between A. Tsaoko And Its Related Plant(A. Paratsaoko)

Objective:Amomum tsaoko Crevost et Lemaire is a perennial herbaceous plant, which fruits were capsules and was a very common materia medica in China. By SSR molecular markers, to get the SSR polymorphism of primers of A. tsaoko, and to acquire the information on the genetic diversity of A. tsaoko, which laid the foundation for selection fine varieties of A. tsaoko. By ITS analysis to search the molecular identification beween A. tsaoko and its related plant (A. paratsaoko), which laid the foundation for the molecular identification of its materia medica. Methods:(1) Microsatellite-markers (simple sequence repeats, SSRs) were widely used in population genetic analysis and genetic mapping due to the high variability caused by changes in their repeat numbers. The genomic microsatellite in A. tsaoko was isolated by magnetic beads enrichment, building the library of microsatellite of A. tsaoko, and getting the microsatellite sequence of A. tsaoko, analysing and designing primer by Primer Primer5.0, then detecting polymorphism of the primers by polyacrylamide gel electrophoresis, and chosing the polymorphic primers of A.tsaoko for its analysis of genetic diversity.(2) The phylogenetic tree was built by ITS molecular markers, and by comparison of their ITS sequence between A. tsaoko and A. paratsaoko, which revealed the genetic differences between them. Results:Of the35individuals in the5populations, the15alleles were detected by the7polymorphic primers of SSR.The15alleles were detected by the7polymorphic primers of SSR. Based on the15alleles, the results of the genetic diversity was got and indicated:the observation heterozygosity (Ho) varying during0.0000～0.4000, and the average of0.3020; the expected heterozygosity value (He) varying from0.2273to0.6704, with an average of0.3936; the Shannon diversity index changing during0.3837～1.0895, and the average of0.6012, the average of Nei’gene diversity was0.3879.(2) A. tsaoko and A. paratsaoko were divided respectively into two clades by ITS anlysis. Conclusions:The genetic diversity of A. tsaoko wasn’t rich. A. tsaoko and A. paratsaoko were accurately distinguished by ITS sequence analysis, which based the foundation for the molecular identification the commercial materia medica of A. tsaoko and the confused materials.