The Experimental Study Of Serum Of Qianlie Tongqiao Capsule On The Smooth Muscles In Vitro Of Urinary System Of Rabbits

Objective： To study the effects of pharmacological serum of QianlieTongqiao Capsule on the detrusors and sphincter muscles of urinary bladder in vitroof rabbits by serum pharmacological methods and investigate the possiblemechanisms.Method: Pharmacological serum of Qianlie Tongqiao Capsule was prepared andstrips in vitro were suspended in the tissue chambers, activity of them was measuredvia Powerlab biological signalling. The effects of pharmacological serum of QianlieTongqiao Capsule in various concentration and those of various blockers on themwere observed. To investigate the possible mechanisms.Result:1、Drug-containing serums prepared after3days,5days and7days ofconsecutive feeding with Qianlie Tongqiao Capsule (3D,5D and7D serum) can allenhance the contraction amplitude, frequency and activity of detrusor, and reducetension of sphincter. Compared with the control group and the blank serum group，the difference was significant for7D serum（P <0.05）. Compared with3D serum,7Dserum was significant difference in effects on bladder muscle （P <0.05）.2、Compared with blank serum group,7D serum in lower, medium and highconcentration (L-, M-and H-serum) can all enhance the contraction amplitude,frequency and activity of detrusor, and reduce tension of sphincter. Compared withblank serum, the M-and H-serum both show significant difference in effects onbladder muscle （P <0.05）. Compared with blank serum group, Qianlie TongqiaoCapsule-containing serum group and Longbishu Capsule-containing serum group inthe same concentration can both enhance the contraction amplitude, frequency andactivity of detrusor, and reduce tension of sphincter. And the difference wassignificant （P <0.05）. The difference between the two groups was not significant（P>0.05）. 3、Compared with M-serum, Verapamil can block the contraction effects ofQianlie Tongqiao Capsule-containing serum on detrusor, and the difference wassignificant（P<0.05）. Qianlie Tongqiao Capsule-containing serum can reduce thecontraction effects of Norepinephrine on sphincter, and the difference was significant（P<0.05）；Verapamil can enhance the relaxation effects of Qianlie Tongqiao Capsule-containing serum on sphincter, and the difference was significant（P<0.05）.Conclusion: With increasing days of drug-feeding, there were increasingeffects of Qianlie Tongqiao Capsule-containing serum on enhancing the contractionamplitude, frequency and activity of detrusor, and reducing tension of sphincter. Theeffects on detrusor and sphincter from M-and H-serum groups in differentconcentration were significantly different. The effect of Qianlie Tongqiao Capsule-containing serum on detrusor may be to play a role through the L-type calciumchannel, and effects on sphincter may be to play a role through theα1receptors. Itwas demonstrated that: Qianlie Tongqiao Capsule can enhance the activity of thedetrusor and reduce the tension of sphincter, in favor of excreting urine. It cansignificantly relieve the clinical symptoms of the detrusor contraction dysfunction dueto Benign Prostatic Hyperplasia (BPH), including weak urination, slow urination anddribbling urine. etc.