Research On The Therapeutical Effect In Transplantation Of Microencapsulated IPCs Into Pancreas Of Diabetic Mice

ObjectiveThe rat pancreatic extract (RPE) was used to induce the bone marrowmesenchymal stem cells (BM-MSCs) into Insulin producing cells (IPCs),thenthe IPCs were microencapsulated and transplanted into the pancreas of type Idiabetic mice.The general state and biological effects were observed.MethodsPurified BM-MSCs were obtained by adherent method.The RPE was usedto induce BM-MSCs into IPCs.The IPCs were identified by immunocytochemi-stry and dithizone (DTZ) stainning. Alginate-PMCG-Alginate(APA) was used tomade microcapsules,then the IPCs were wrapped and detect energy. Diabeticmice models were produced by injected streptozocin (STZ).Diabetic mice wererandomly divided into four groups: A:sham-operation group; B:BM-MSCsgroup; C:none microencapsulated IPCs group; D:microencapsulated IPCsgroup. Grafts were transplanted into the capsule of the pancreas. Weight、blood glucose、 insulin and C peptide were monitored dynamicly.Aftertransplantion for50days,the mice were executed, immunofluorescence wasused to observe the islet tissue.Results1．The BM-MSCs became round and gathered to grow in groupsinduced by RPE, Dithizone and immunofluorescence staining waspositive,which indicated the cells could secreted insulin. 2．Microcapsules and cells compatible well.But microcapsules didn,taffect the secretory of the cells.3．Result of body weight and biochemical parameters:(1) body weight:Group A sustained reductions.Group B and C began to rise at3d fromtransplantation, but decreased at30d from transplantation. Group Dcontinued to rise after transplantation.(2) Glucose: Group A continues to rise.Group B and C began to decrease at3d from transplantation,but rise at30dfrom transplantation. Group D continued to decrease after transplantation.(3)insulin and C-peptide: At50d from transplantation, insulin and C peptide weresignificantly increased in group B, C, D,group D is the most obvious.4．The results of light microscopy:50days after transplantation, group B,Cmouse pancreatic see the isletβ-cells decreased the volume reduced.Group D of the pancreas can be seen implanted microcapsules,themicrocapsules wayward good there is no damage visible to insulin-like cellswithin the microcapsules.5．Result of immunofluorescence:weak green fluorescent protein couldbe seen in group B and C after50days from transplantion.More greenfluorescent protein could be seen in the pancreas in group D, and around themicroencapsules was more obvious,which suggested that the function ofendocrine insulin of microencapsulated IPCs could maintain a longer time.ConclusionsThe microencapsulated IPCs transplanted into diabetic mice pancreas,they have played a role in elevating body weight, insulin and C-peptide andlowering blood glucose. Microencapsulated IPCs transplantated into thepancreas may had the function of treated DM.