Expression And Clinical Significance Of MMP-9and CD133Protein In Human Gliomas

Objective:Glioma is the most common malignant tumor in brain tumor, it showedinvasive growth, easy to relapse, and more for local recurrence, rarely distantmetastasis.surgery, radiation, chemical and genetic drug treatment are difficult to cure.MMP-9can degrade extracelluar matrix which surrounding brain tumor cells, cancer stemcells can make use of MMP-9degradation, destruction of tumor cells in the ECMcollagenase, and invasion nnormal and distant metastasis tissue along the basement. Thistopic is aimed at the detection of MMP-9, CD133protein expression in human gliomas,and analysis the relationship in the pathological grade of glioma between matrix MMP-9and CD133protein, inorder to provide a basis for judging giloma prognosis、takeprecautions againstand treatment strategies.Methoed:Divided into two parts:(1)The first experimental group, select the JiningCity, Shandong Province, People’s Hospital of Neurosurgery, January2009to July2011,66cases of glioma specimens.The second experimental group:select the Jining City,Shandong Province, People’s Hospital neurosurgeon from March2006to September2011,24cases of recurrence in patients with glioma specimens. In accordance with theclassification and grading standards of the WHO (2000edition).The specimens were fixed by10%formalin. and paraffin-embedded. Control groupwere normal brain tissue.fixed as above.all the specimens were use two-step TM. WithPBS instead of primary antibody as blank control, normal brain tissue as a control. MMP-9protein was mainly localized in the cytoplasm where shows brown means in the positive expression. CD133protein was localized in the cell membrane.where appear brownparticles in the backgroundm means positive expression. Scores can range Hilbe methodswith reference to the randomly selected four high-power field (×400), count thepercentage of positive cells accounted for the total number of cells in the field of vision.Criteria were: not found positive cells (-), the number of positive cells accounted for25%of the total number of cells as (+),25~50%(++),51~75%as (++),75%or more of (++++), respectively, divided into0,1,2,3,4points in SPSS17.0statistical software for dataprocessing, results, results were selected according to the data type, the nonparametric testand Spearman rank correlation analysis, inspection level α=0.05.Result:Experimental one (1) is not detected MMP-9, of CD133protein expression in15cases of normal brain tissues.(2)66cases of positive expression rate of CD133proteinin human glioma specimens average of23.8511±27.77105(%), MMP-9protein positiveexpression rate of an average of30.0877±29.55121(%).(3) CD133and MMP-9protein inthe total positive expression rate on application spearman rank correlation analysis: alongwith the increase in the level of Pathological increased (p<0.01).③human glioma CDl33protein positive expression rate of MMP-9protein positive cells express the rate ofapplication grade spearman correlation analysis: both a positive correlation (p<0.01).Experiment2(1) we did not detected MMP-9and CD133protein expression in15cases ofnormal brain tissues.(2)24cases of glioma specimens with two times of surgical., thepositive expression rate of CD133protein in primary glioma specimens was30.5±30.0(%), recurrent glioma specimens positive expression was47.6±30.1(%), There are greatdifference in primary and recurrent gioma (p<0.05).(3)24cases of glioma patients withtwo times of surgical specimens, The positive expression rate of MMP-9protein in primaryglioma specimens was38.0±28.4(%). In recurrent glioma specimens The positiveexpression rate was55.4±28.3(%), the difference was statistically significant (p<0.05).(4)CD133and MMP-9protein expression on strength applications spearman rank correlationanalysis: in primary, recurrent glioma with the increase of Pathological Grades of higher(p<0.01).(5)24cases of glioma patients with two surgical specimens, recurrent glioma pathology level than primary increased in eight cases, pathological grade of the same16cases. Visible recurrence after pathological level there is an increasing trend (p<0.01).(6)CDl33protein positive expression rate of MMP-9protein positive expression rate inprimary and recurrent glioma in a positive correlation (p<0.01). There is a closerelationship between the two.Coclusion:1.We detected MMP-9and CD133protein highly expressed in human gliomaspecimens, and was not detected MMP-9and CD133protein expression in normal braintissue.2. We found MMP-9protein and CD133protein expression was significantly higherthan I, Level II (low level).3. Glioma recurrence shows much more inwsaive than before.4. The result shows that the expression of MMP-9and CD133related to pathologicalgrade in primary or recurrent glioma.5. Recurrent brain glioma specimens CDl33and MMP-9protein positive expressionrate was higher than primary brain glioma6.There is a positive correlation about the expression of CDl33and MMP-9in both theprimary or recurrent glioma.