Study On Rats With OSAS And Ischemic Stroke

Object: Through the establishment of the rats with obstructive sleep apnea syndrome (OSAS) model and the involvement of high blood lipid, high blood glucose factors, this paper aims to discuss pathophysiological mechanisms in OSAS and its correlation with ischemic stroke.Methods:Wistar rats are randomly divided into six groups:normal control group, hyperlipidemia group, diabetes group, OSAS group, OSAS+hyperlipidemia group, and OSAS+diabetes group. Establish OSAS model through the injection of sodium hyaluronate gel in the rats’pharynx multi-points, and monitor OSAS rats’EEG, oronasal airflow and dynamic oxygen for2hours, the model is determined to be successful if there are differences in the mean oxygen saturation, lowest oxygen saturation and sleep apnea index statistics before and after the model establishment.①Use automatic biochemical analyzer to measure serum C-reactive protein (CRP) levels, and use automatic coagulation analyzer to measure plasma fibrinogen (Fbg) levels,(2) Use ELISA method to measure plasma homocysteine (Hcy) level;③Use HE staining to observe the rats’ cortex and hippocampus structures.Results:①The mean oxygen saturation (89.75±2.01), lowest oxygen saturation (72.77±5.19) of OSAS rats reduced, and sleep apnea index (5.70±1.02) increased, there are statistically significant differences (P<0.01) compared with the one before modeling.②CRP (15±0.04), Fbg (2.46±0.90) of OSAS group increased compared with normal control group, negatively correlated with the mean oxygen saturation (r respectively is-0.802,-0.867, P<0.01), and positively correlated with sleep apnea index (r respectively is0.874,0.941, P<0.05); CRP (15±0.04), Fbg (2.78±1.50) of OSAS+hyperlipidemia group increased compared with normal control group, negatively correlated with the mean oxygen saturation (r respectively is-0.901,-0.963, P<0.05), and positively correlated with sleep apnea index (r respectively is0.933,0.975, P<0.05); CRP (0.16±0.03). Fbg (3.27±1.83) of OSAS+diabetes group increased compared with normal control group, negatively correlated with the mean oxygen saturation (r respectively is-0.881,-0.869, P<0.05), and positively correlated with sleep apnea index (r respectively is0.928,0.990, P<0.05).③Hcy (10.94±2.48) of OSAS+hyperlipidemia group increased compared with the other five groups, positively correlated with the sleep apnea index (r is0.867, P<0.05).④There are neuron loss, disorganization and small vessel proliferation in HE staining cortex and hippocampus of the brain tissue in OSAS group, OSAS+hyperlipidemia group, OSAS+diabetes group have more significant changes compared with OSAS group.Conclusion:①The levels of CRP, Fbg, and Hcy of OSAS rats increase, and are closely related to OSAS severity. Inflammatory response increasing, blood hypercoagulability and hyperhomocysteinemia of OSAS rats may be the pathogenesis of ischemic stroke resulted from OSAS.②There are performances of cortex and hippocampus neuron loss, disorganization and small vessel hyperplasia in OSAS rats.