| Objective:Comparison of sodium pyruvate, and PD-2intraperitoneal resuscitation of intestinal mucosal barrier protective effect of hemorrhagic shock in rats, serology, morphology, and protein levels of sodium pyruvate intraperitoneal resuscitation on hemorrhagic shock in rats intestinal mucosal barrier functionprotective effect and its advantage in the recovery of the abdominal cavity.Methods:Healthy and clean male SD rats,40were randomly divided into four groups: sham operation (control group), the model of hemorrhagic shock group (shock group), the compound sodium chloride (Ringer) vein recovery+the PD-2fluid peritoneal fluid resuscitation group (PD-2liquid group), the compound sodium chloride intravenous recovery (Ringer)+sodium pyruvate solution intraperitoneal resuscitation group (pyruvate group)(n=10). The control group followed the line of the right common carotid artery, the right femoral vein, left femoral artery and systemic heparinization. Shock in the control group on the basis of the left femoral artery and bled, and preparation of hemorrhagic shock rat model. PD-2fluid group, sodium pyruvate group in the shock group on the basis of the right femoral vein recovery, recovery of the liquid for the release of blood and two times the amount of Ringer’s solution. To the intraperitoneal injection of20ml2.5%PD-2solution with20ml of sodium pyruvate solution. The2h drawn in the control rats intubation (arterial and small intestinal specimens) tested; shock rats1h drawn after the model; the PD-2medium group, sodium pyruvate rats1hour after resuscitation drawn. Keep the rats free unobstructed breathing, heat lamp to maintain body temperature of rats at37±0.3℃. Take the left femoral artery in the experimental design time, an automated blood gas analysis system for determination of arterial blood gases (pH,PaCO2,PaO2). Spectrophotometric determination of plasma D-lactate (D-lactic acid, D-LA) content, light microscope, were observed by the histological changes of the rat small intestine. Determination of small intestinal epithelial phosphorylation of vasodilator stimulated phosphoprotein (p-the VASP) and downstream tight junction structural protein ZO-1expression by Western blot and immunohistochemical methods.Results:Blood gas analysis:pH and PaO2level of the shock group than the control group decreased significantly, the difference was statistically significant (P<0.01), PaCO2was lower, the difference was not statistically significant (P>0.05); the PD-2medium group, sodium pyruvate significantly higher in group than in the shock group, pH levels and PaO2content, the difference was statistically significant (P<0.01), PCO2increased, the difference was not statistically significant (P>0.05), compared with the PD-2medium group, sodium pyruvate The group pH levels and PO2levels occur more obvious differences between the two groups was statistically significant (P<0.05); PCO2of change was no significant difference (P>0.05). D-LA content of the results:the shock group than in the control group comparison, D-LA content was significantly increased, the difference was statistically significant (P<0.01); PD-2medium group, sodium pyruvate group than in the shock group was significantly lower, the difference statistically significant (P<0.01). Comparison with the PD-2fluid group, sodium pyruvate group D-LA declined more significantly, there was significant difference between the two groups (P<0.05). Light microscope, the control group, the rat small intestine layer of fluff arranged in neat rows, no edema, submucosal vascular filling. Layer of shock group, the rat small intestine villi height of edema and vascular collapse, submucosal glands focal area of necrosis in the lamina propria, and large areas of mucosal villi necrosis, loss, defect; small intestinal mucosal changes of the PD-2medium group and the sodium pyruvate group compared with shock group was significantly reduced, see mild edema of the small intestinal mucosal layer of fluff, a small amount of necrosis, loss, mild submucosal blood vessels collapse; sodium pyruvate group in the rat small intestinal mucosal villous edema, lodging and the degree of necrosis was better than the PD-2liquid group, submucosal blood vessels than the filling. Intestinal epithelial damage index score sodium pyruvate group than in the shock group and the PD-2medium group (P<0.01or P<0.05). Western blot results showed that the shock group, the PD-2fluid group, sodium pyruvate group the rat small intestine of ZO-1protein levels compared with the control group (P<0.01), p-the VASP protein levels than the control group (P<0.01); compared with shock group, the PD-2medium group, ZO-1protein content of the sodium pyruvate group of the rat small intestine were significantly higher (P<0.01), p-the VASP protein content decreased (P <0.01), which ZO-1protein content in the small intestinal mucosa of sodium pyruvate group increased more significantly (P<0.05), p-the VASP protein expression significantly decreased (P<0.05); the sodium pyruvate group compared with the control group the difference was not statistically significant (P>0.05). The immunohistochemical results of each group is consistent with the Western blot.Conclusion:1. An intravenous resuscitation and abdominal recovery can significantly reduce the hemorrhagic shock in rat plasma D-LA content, increased PO2level, help to correct the acidosis, and improve the oxygen partial pressure. In antioxidant capacity and the correction of acidosis, sodium pyruvate is better than the PD-2fluid. The experiment may prove to be stable hemorrhagic shock in rat small intestinal epithelial morphology, inhibition of intestinal flora shift to achieve the protective effect on intestinal mucosal barrier.2. Hemorrhagic shock in intestinal epithelium of p-the VASP expression was increased, decreased expression of tight junction protein ZO-1; abdominal recovery after reducing the expression of p-VASP, the stable expression of ZO-1in intestinal epithelial, and thus play the intestinal mucosa barrier function. |
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