Combind Effects Of Sodium Cyanide Intoxication And Hemorrhagic Shock On Cardiac Function In Rabbits

Cyanide is a hypertoxic compound widely used in industry and militqry forces in the world. Hemorrhagic shock, the most severe syndrome, in the battle injuries can result in a higher fatality rate. Combind injuries of chemical intoxication and wound have been reported frequently in accidents or disrasters. However, no detailed research on the combind effects of sodium cyanide intoxication and hemorrahgic shock and their treatment was seen up to now.Object: Physiological,biochemical and molecular biological techniques would be used to determine the interaction between hemorrhagic shock and cyanide intoxication. Further studies were carried out to clarify the influences of the combind injuries on cardia function and oxdative stress.Methods: Combind injury animal models were established in rats and rabbits. The function of respiration and circulation were recorded with electrophysiological system. Myocardium enzymogram and blood gas were analyzed biochemically. Spectrophotometry was employed to detect SOD,MDA and ROS in serum and NO and iNOS in cardiac muscle. Western blot were also used to further the study of iNOS.Results: The LD50 value of sodium cyanide decreased obviously in the animals with combind injuries. There was an extention in Q-T stage .Cardiac muscle zymogram and blood gas were changed. The value of PH,PaO2 and HCO3- decreased manifestly. The activity of SOD decreased, however, iNOS activity and the amount of MDA,ROS and NO increased. There were a significant difference among intoxication group,hemorrhagic shock group and combine injury group.there was a amelioration after the use of 4-DMAP.Conclusion: Hemorrhage and hemorrhagic shock elevated the toxicity of sodium cyanide and improve its injury to cardiac function. Disorder in the course of cardiac muscle repolarization and obvious increase in the amount of cardiac muscle enzymes can cause...