Analysis On Differences Of Gene Expression Profiles Of Kidney-Yang Deficient Physique At Different Age Segments And Verify Genes In Old Group With PCR

Objective:Based on intergroup analysis about the differentially expressed genes of Kidney-Yang deficiency (KYD) physique at different ages, to discuss differentially expressed genes in young, mature, and old aged groups, verify the differentially expressed genes related to senile KYD syndrome with PCR and provide advices on life prevention and cure to the KYD physique.Methods:1. Review literature about KYD and analysis academic view;2. Use the SAM3.1which is developed by Stanford University to analysis differentially expressed genes from the earlier gene expression information;3. Discuss the gene regulation mode of KYD in young, mature, and old aged groups from differentially expressed genes、gene functional classification and molecular pathway;4. Select elderly patients with KYD which meet up with KYD physique diagnosis standard and healthy people for control;5. Use the skill of the PCR to verify the differentially expressed genes related to senile KYD syndrome;6. Analysis the clinical significance and therapeutic principle on improving senile KYD syndrome from differentially expressed genes related to senile KYD syndrome.Results:1. The ancient and modern literatures reveal the research on KYD physique is valuable and instructive for preventing diseases and health caring;2. Screening on differentially expressed genes, we gained11differentially expressed genes in young,10differentially expressed genes in mature,3differentially expressed genes in old;3. The genes expression character at different ages explains differentiation on syndrome and sign from a certain extent;4. The PCR verify that3differentially expressed genes in old are reliable. Discuss:1.The difference exist in KYD physique at young, mature, and old aged groups, the young focus on facilitation about growing and relationship with lung and kidney, the mature focus on regulation about qi-blood-body fluid and relationship with liver and kidney, the old focus on supporting about kidney and relationship with brain and kidney.2. The gene expression character at different ages reveal the age related spatiotemporal principles about KYD, so we should know the spatiotemporal feature in clinical;3. Improving and nursing the senile KYD physique may slow down ageing. Warming Kidney Yang is the basic principle and main therapeutic for improving the senile KYD physique and slowing down ageing.