Expressionand Clinical Significance Of EphA2/E-cadherin Inpatientswith Endometrial Carcinoma

BackgroundEndometrial Carcinoma (EC) is one of the three common malignant tumors in women,and the incidence of this disease has risen to number four in female malignant tumors afterbreast cancer, colorectal cancer and lung cancer [1]. Especially in recent years, theincidence rate and mortality rate of EC have been accelerating worldwide. There are4,200people in America who are diagnosed with EC, which is the number one in terms ofincidence cases in women genital tract tumor. The survival rate is only85%in5years[2-3]. As we are not clear about the pathogenic causes and mechanism of CE molecules’biological base, and the early detection and prognosis are not perfect, so the study of EC’sgene biology and molecular biology targeted treatment have become a new direction. Wecan hence provide an effective treatment scheme of molecular biology for the sickwomen’s later years and recurrence of the disease. It is very important for an earlydiagnosis and treatment of EC, so early diagnosis and treatment from gene level hasbecome a hot research topic in many big institutionsObjectivesThrough checking the expression differences of EphA2and E-cadherin in normalendometrial tissues, atypical endometrial tissues and endometrial carcinoma tissues, theauthor explores the expressions and clinical significance of EphA2and E-cadherin in EC.Method1．Detecting the expressions of EphA2and E-cadherin in35cases of normalendometrial tissues,35cases of atypical endometrial hyperplasia tissues and50cases ofendometrial carcinoma tissues by means of immunohistochemistry (SP); defining theiraccurate expression location, and making a statistical analysis of the expression rate ofpositive cells. 2． Detecting the contents of EphA2and E-cadherin in endometrial carcinoma tumortissues and non tumor tissues with reverse transcribing polymerase chain reaction(RT-PCR) technique, and making a semi-quantitative analysis of the expression differencebetween them.3． Detecting the contents of EphA2and E-cadherin in endometrial carcinoma tumortissues and non tumor tissues with Westenn blot, and making a semi-quantitative analysisof the expression difference between them.4．The expressions of EphA2and E-cadherin are examined in35cases of normalendometrium,35cases of atypical hyperplasia endometrium and50cases of endometrialcancer tissues by RT-PCR, immunohistochemistry (SP) and western blot method. Andthe expression differences were analyzed by SPSS13.0。Results1. With immunohistochemistry, normal endometrium has been confirmed thefollowing:(1) The strong positive expression rates of EphA2are98%、2.85%、17.14%respectively in cancer tissue group (50cases of EC), non-tumor group (35cases of normalendometrial tissues), and atypical hyperplasia endometrium tissues (35cases). EC grouphas increased significantly. The comparison of the three has statistical significance(p<0.05). EphA2is related to the histologic grading of EC, with or without myometrialinfiltration and operation-pathology staging (P <0.05).(2) The strong positive expression rates of E-cadherin are respectively4.00%、85.71%、91.42%in cancer tissue group (50cases of EC), non-tumor group (35cases ofnormal endometrial tissues), and atypical hyperplasia endometrium tissues (35cases).E-cadherin in the EC group has decreased significantly. The comparison of the three hasstatistical significance (p<0.05). E-cadherin is related to the histologic grading of EC, andoperation-pathology staging (P <0.05).(3) The expressions of EphA2and E-cadherin in EC in are negatively correlated (rs=-0.694, P <0.05).2. The following has been obtained by means of reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blot,EphA2gene expressions are high on average in detecting endometrioidadenocarcinoma; E-cadherin gene expressions are weak in atypical hyperplasia ofendometrium and normal endometrium group. 3. A comparative analysis of immunohistochemistry, reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blot:Comparing the results of EphA2and E-cadherin respectively by means ofimmunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR) andWestern blot, and the results are considered the same from the three kinds of detectionmethods (P>0.05).Conclusions1．The strong positive expression rate of EphA2is high in uterine tissues and atypicalhyperplasia of endometrium, which is related to the histologic grading of EC, with orwithout myometrial infiltration and operation-pathology staging; E-cad expression is lowerin EC than in normal endometrial tissues, which is related to histologic grading of EC andoperation-pathology; EphA2and E-cadherin play important roles in the occurrence anddevelopment of EC.2．EphA2and E-cadherin are closely related to the local infiltration and distantmetastasis of EC; the increasing expression of EphA2or the decreasing expression ofE-cad may indicate a higher degree of malignant tumor or a poor prognosis.3． The abnormal protein expressions of EphA2and E-cadherin show that they areboth involved in the occurrence, development and metastasis of EC. Detection of the twoproteins jointly has some reference value in evaluating the prognosis of EC.