Study On Chemical Composition Of Stems And Leaves Of Scutellaria Baicalensis Georgi

Scutellaria Baicalensis Georgi is one of Chinese traditional medicine which is labiataeplant, it is mainly produced in the north of the country, and its root has much medicinal effect.Wild resources is difficult to meet people’s needs, because of arbitrary excavation year by year,Scutellaria Baicalensis Georgi is effectively compensate for the lack by largely planted inlarge area, but a large number of stems and leaves have been discarded. Since the early1980s,people have began the experimental study of the pharmacological activity in the stems andleaves of Scutellaria Baicalensis Georgi. The Pharmacology study proved that it had strongeffect on anti-virus, anti-inflammatory, anti-oxidation, reducing blood lipid, relatively strongmemory improvement. In this paper, the research focuses on extraction and separation processof the flavonoids, analysis and identification the chemical structure of the compounds basedon Scutellaria Baicalensis Georgi. The purpose is to explore the optimum conditions of theflavonoids extraction and separation, and the structure was analyzed and detected by moderninstruments.First of all, the extraction process of total flavonoids from Scutellaria Baicalensis Georgiwas studied through repeated experiments. Water extraction is used in adjusting the PH, themacroporous adsorption resinand and desorption in extraction process of total flavonoids. Theresults show that the optimal extracting conditions is: ratio of material to liquid,1:25(w:v),extracting time,2hours, hydrochloric acid to adjust the extract PH1～4，supernatant after centrifugation, adsorption by pretreated macroporous resin (AB-8),95%ethanol desorption, flow rate,2mL/min.Second, this study uses the separation of the traditional methods and modern separationtechniques, including Macroporous resin, polyamide column chroatography atmosphericpressure, preparative TLC, preparative high performance liquid chromatography and otherpurification techniques. Polyamide thin layer experiments as a guide, thin layer conditionswere as follows: developing solvent dichlormethane: methanol, formic acid (50:48:2),reagent ethanol solution of1%ferric chloride, expand the time for about15minutes. Andthen rough separation through polyamide column chromatography from the extraction of thestems and leaf of Scutellaria Baicalensis Georgi. Using different proportions of dichloromethane and methanol（10:1,8:1,5:1,2:1,1:1）wash column, TLC merged streamswereits further purification by Preparative High Performance Liquid Chromatography. In thisprocess, the selection of column was SymmetryPrepTMC18(7.8×150mm,7um), and themobile phase were methanol and0.1%formic acid in water. The separation process wasconvenient, fast, and suitable for the separation and preparation of the stems and leaves ofScutellaria Baicalensis Georgi.Last, the results show that the total flavonoids from stems and leaves of scutellariabaicalensis Gerogi purified in further by water extraction, ultimately5compounds wereisolated from the stems and leaves of Scutellaria Baicalensis Georgi. In order to identify thechemical structure of the compounds, this study with the UV, IR, MS,13CNMR,1HNMRand the physical and chemical nature. In the end, we can know that the five compounds areChrysin7-O-β-D-glucosid, Baicalin, Apigenin-7-O-β-D-glucoside, Scutellarin andCarthamidin-7-O-β-D-glucoside.As a byproduct of Scutellaria Baicalensis Georgi, the stems and leaves of ScutellariaBaicalensis Georgi are rich in China and have great value. This paper studies on the chemicalcomposition of the stems and leaves of Scutellaria Baicalensis Georgi. Five kinds offlavonoids compositon have certified and provided the scientific basis for the development ofnew, effective and safe drug.