Investigation On The Effect Of The Surface Roughness Of The Electrospun Polylactide Fibers On The Cellular Behaviours Of Rat Bone Marrow Mesenchymal Stem Cells

Currently the influence of nanoscale surface topographical features on cell functions attracted more and more attentions. In the field of regenerative medicine, cell-material interaction is a fundermental topic, in which one of the important is that the material surface structure can control the cellular functions. Both the surface topographical structure and the specific nano-effect can influence cell functions such as cell adhesion, spreading, proliferation and differentiation.Firstly, the surface nanoroughness on the nanofiber membrane was engineered with an electrospinning approach by simply adjusting the concentration of polymer solution. The surface topographical features were characterized with scanning electron microscopy (SEM) and atomic force microscopy (AFM). The aspect ratio and the average number of the beads per100um length of a single nanofiber were observed with SEM Photos, and the half-height of beads reflected the surface roughness was characterized with AFM.Secondly, rat bone marrow mesenchymal stem cells (BM-MSCs) was obtained from the10-day-old newborn mice bone marrow, by whole bone marrow culture method, which with medium containing serum anti-Complex washing bone marrow cavity, collects bone marrow cell suspensions, inoculated culture. By changing medium at a selected time to abandon the hematopoietic cells, and passage time was strictly controlled so that rBMSCs were purified.Thirdly, the impacts of different surface nanoroughness on cellular behaviours were further investigated from cell adhesion, spreading, proliferation and differentiation through co-culturing with rat bone marrow mesenchymal stem cells (rBMSCs). The result showed that the existence of beads on the surface of electrospun nanofibers does not bring any negative effect on cell growth, and contrarily the moderate surface nanoroughness from the beads can modulate and facilitate cellular behaviours. Results showed that:certain extent the electrospun nanofibers with suitable nanoroughness can be more great potential for biomedical application as tissue engineering scaffold.Finally, to investigate the effect of the nanoroughness on bone replacement in vivo, the fibers were biomineralized in vitro and then implanted in vivo. The result displayed that the fibers with modulate surface roughness have little inflammation to animals after4weeks of implantation, and they can promote bone bone tissue growth to a certain extent. After, the fibers were degraded into pieces. The result of research in vivo also indicated that the fibers with moderate roughness are suitable for the application as tissue engineering scaffold.