Differential Expression Of MicroRNAs In The Alveolar Bone Of Rats With Traumatic Occlusion Resulting In Tooth Root Resorption

Objective: To study the differential expression ofmicroRNAs(miRNAs) of traumatic occlusion resulting in toothroot resorption with the animal model of rats，and to providethe basis of new molecular targets for the regulation of thebone formation and resorption.Methods: The occlusal surface of the upper left firstmolar of rat was raised by placing a stainless steel wire toinduce occlusal trauma in the lower left first molar. After14days, the alveolar bone tissue of the first molars at theboth sides of rats’lower jaws were taken out under anesthesia.Total RNA was isolated from every sample using trizol. RNAquality and quantity was measured by using thespectrophotometer and RNA integrity was determined by gelcontaining formaldehyde electrophoresis. After RNA isolationfrom the samples,the Genisphere FlashTag power labeling kitwas used according to the manufacturer’s guideline for miRNAlabeling. After stopping the labeling procedure, the labeledsamples were hybridized on the GeneChip miRNA2.0Array(Affymetrix). Then the slides were scanned using the GeneChip Scanner3000. Scanned images were then imported into thesoftware for analysising differentially expressed miRNAs.Results: In the results of the study,28differentexpressions were found, of which the expressions of18miRNAsincreased and10miRNAs decreased.Conclusions:(1)miRNAs were screened by usinghigh-throughput microarray technology, there were28 differential expression of which18up-regulated and10down-regulated.(2)We may find new regulatory genes on the bone formationand resorption from differentially expressed miRNAs.(3)To provide the basis of new molecular targets for theregulation of the bone formation and resorption.