The Research Of Gelatin Induced Enamel-liked Fluorapatite Biomimetic Synthesis On Tooth Surface

The biological material is a medical purpose, for living tissue in contact to formthe function of the non-living materials, and can be divided into two categories of thenatural biological material and artificial biological material. Tooth tissue is the one ofthe typical natural inorganic biological material. Human tooth enamel is the outermostlayer and the most hard mineralized tissue in the body, and there are not living cells inmature enamel different from other calcified tissue (e.g., dentin and bone), becausethe enamel ameloblasts disappeared after enamel formed, so that no cells can repairthe enamel when it is damaged. Therefore, the study of the synthersis of mimeticenamel column structure via the non-biological methods in vitro is still a trend of oralbiomimetic materials research. Biomimetic mineralization is a method to synthesizethe artificial lens of simulated biological mineralization process. Biologicalmineralization of the inorganic mineral is often proceed in the conditions of theorganic matrix existing, which will control the nucleation, growth and orientation ofbiomimetic enamel structure. The study has important clinical significance, which thebiomimetic self-healing repair of tooth structure use the method of biomineralizationinduced by the organic macromolecules as template after dental tissue injuried,according to the principle of the regulation of inorganic growth under the controlling of the organic matrix, the paper from the following studies:1, In this paper, the biomimetic materials, fluoro-apatite (FA) crystal with theenamel-like structure, were synthesized under the near-physiological conditions. Thechange of crystalline morphology was investigated under different reaction time, andthe best size and morphology of the crystals were obtained. The results are as follows:With the extension of the reaction time, the morphology of the crystals changed fromthe spherical particulate to hexagonal prism-like rod-like crystals clusters, with thecross-sectional diameter of about1μm, a length of about6~7μm.2, Based on the above work, the ordered hexagonal prism enamel-likefluorapatite crystals were synthesized under the organic macromolecular gelatin wasas template, and the effection of reaction time on the morphology and arrangementof crystal were studied. At the same time, the morphology, structure andcharacterization of the re-synthesized crystals were investigated by the scanningelectron microscopy (SEM), X-ray powder diffraction (XRD), Fourier transforminfrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS).3, The ordered enamel-like fluorapatite crystals were synthesized on the enamelsurface under near-physiological conditions (37℃,1atm, pH6.0), based on thegrowth law of the fluorapatite crystal. The results showed that the single free-standinghexagonal prism-like crystals gathered to the bunch, with the almost same size, andthe growth was along the vertical direction of the enamel surface. The size of cross section is approximately300nm and it is about6-7μm along the c-axis.4, The physical chemical and biological properties of the synthetic sample werestudied, the results show that: the chemical composition and the surface morphologyof the crystal film are simily to the natural enamel and has good biocompatibility andantibacterial properties.