Biomimetic Remineralization Efficacy Of An Amelogenin-based Synthetic Peptide On Caries Lesions

ObjectiveThe aim of this study was to evaluate the remineralization efficacy of an amelogenin-based peptide on early enamel lesions in vitro.Our study could provide basic for exploring the new biomimetic remineralizing agents which can promote early enamel lesions to form biological multigrade structure.MethodsInitial caries lesions on smooth enamel surfaces of sound bovine incisors were created using a previously reported demineralization model.Specimens were randomly assigned to five treatments(n = 10)with an immersion model for 12 days: A,the remineralizing medium;B,12.5 μg/mL synthetic peptide;C,25 μg/mL synthetic peptide;D,50 μg/mL synthetic peptide;and E,fluoridated remineralizing solution.All peptide treatments were carried out in remineralizing medium,and the remineralizing media was replenished every 2 days.After immersion,mean mineral loss before and after remineralization of each specimen was determined using micro-CT.Mean mineral gain after remineralization and the percent remineralization of each specimen were calculated.ResultsA significant decrease in mineral loss after remineralization process in all groups was observed(p<0.05).Enamel lesions treated with the fluoridated remineralization solution had a highest mineral gain among all treatment groups.Inclusion of synthetic peptide in the remineralizing solution at concentrations of 25 or 50 μg/mL exhibited a higher mineral gain compared to remineralization solution only.No significant difference was observed between the remineralizing medium treatment and the 12.5 μg/mL peptide treatment in mineral gain(p>0.05).Mineral gain of amelogenin-based synthetic peptide treated groups was observed to be dose-dependent.And the analyzed results of the percent remineralization were consistent with the results of mean mineral gain.ConclusionsOur study confirmed that specific concentrations of the amelogenin-based synthetic peptide promoted in vitro remineralization,with higher concentrations exhibiting significantly greater remineralization.