Studies On Extraction And Separation Of Active Constituents And Anti-Prostatic Cancer Activity In Vitro Of Baoxing Saxifrage (L) Meeb

The purpose of this study was to extract the active constituents from Saxifrage in Sichuan Baoxing Area, and preliminary research on its inhibition of DU145androgen-independent human prostate cancer cells in vitro and induction of apoptosis.The experimental methods and results are as follows:1. The extraction and separation of active constituents of Saxifrage:The fresh dry Saxifrage was treated by water decoction and alcohol precipitation firstly, extracted with ethyl acetate; and then separated and purified the extracts by silica gel column chromatography. Selected the optimal conditions of column chromatography, separated on the column and collected the segmental fractions, marked as F1, F2, F3, F4, F5, F6, F7,and F8for spare.2. MTT Method:To determine the selected segmental fractions inhibit DU145cell proliferation by MTT assay. After320mg/L,160mg/L,80mg/L,40mg/L,20mg/L drug respectively treated DU145for24hours,36hours and48hours, the effects of F1and F8were not obvious, the rest of the fractions were varying in degree of inhibition of cell proliferation. Among those, F3, F6, and F7section of the dose-response relationship and time-effect relationship were the most significant, with IC50values were respectively55.63mg/L,50.02mg/L, and102.2mg/L.3. Cell apoptosis detection: Selected F7as research object, to determine its effect of DU145cell apoptosis under24hours,36hours, and48hours. The result showed that the F7can promote the cancer cells apoptosis, and positively related to the dose-response relationship and the time-effect relationship.These results demonstrate that the active constituents of Saxifrage separated by silica gel column chromatography can inhibit DU145cell proliferation in vitro and promote its apoptosis. It is an active material library of anti-prostate cancer.