Extraction Separation Of Velvet Antlers Polypeptide And Promoted Proliferation Of Osteoblast Cell

Deer velvet antler is the young antler which is still not ossified and full of velvet of the male deer Cervus Nippon Temminck. Velvet antler is one of the most popular natural tonics and traditional medicinal substance. It is a valuable drug in the Orient.Velvet antler contains numerous regulatory substance, chemicals and co- mponents, such as polysaccharide, phosphatide, hormone, mineral composition, polypeptide and protein.The growing antler has been used for the treatment of various diseases in oriental medicine. Deer antler as an animal source of the Chinese tradition medicine has been used in therapy of neurosis, enriching the vital energy, nursing the blood, strengthening the kidney and prolonging life for thousands of years. Many reports made it evident that velvet antlers have various effects: anti-inflammatory affects anti-whiplash activities as antistress activities and anti-aging activities, etc. A water extract of velvet antler which prepared from the velvet antler of sika deer (Cerv us Nippon Temminck). Extract has been widely used in the treatment of some osteocyte-related diseases, such as osteoporosis, osteoarthritis, and bone fracture.People with osteoporosis(OP) experience a low bone mass and a weakening of the bone structure . This can eventually result in debilitating fractures, particularly of the hip, spine and wrist. The reason of OP is general consider that the body is short of calcium and vitamin, endocrine disturbance, nutritional disturbance and lack of sports and illumination. Most people currently suffer from osteoporosis,and another's are considered to be at risk for the disease. Women are four times more likely than men to develop the bone loss condition. Approximately 30%-50% for female and 15%-30% for male will suffer from bone fracture which initiation by osteoporosis. Osteoporosis is dividatur into 3types: primarily osteoporosis, continue osteoporosis and idio- osteoporosis.The native total peptides(VATP)were extracted from velvet antlers of sika deer ( Cerv us nippon Temminck). A high active peptide component (VAP-B)stimulating proliferation of human osteosarcoma cells was isolated from VATP. Finally, a further purified active peptide component (VAP-B1)with molecular weight distribution from 200-800 Da was obtained from VAP-B by preparative HPLC. VAP-B1 is able to enhance the average content of DNA in S phase. And also the alkali phosphatase (ALP) activity in osteosarcoma cells increased obviously with an increase in VAP-B1 dosage and treatment time. These results are consistent with cell cycle variation and ALP level as a marker of bond formation during osteocyte proliferation and differentiation, as well as maturity. This study provided a clue to explore a new peptide medicine from velvet antlers for therapy of osteoporosis.