| Cichorium intybus L.(chicory)(Compositae) is a perennial herb growing in northwest of P. R. China. The whole plant, listed in the book’Zhong Guo Min Zu Yao Zhi’, was used as a Traditional Chinese Medicine. It tastes a light bitter and cool in nature. Modern pharmacological research showed that C. intybus could improve functions of heart and digestive organs. It also exhibited hepatoprotective, fat-reducing, anti-hyperglycemic, and antibacterial activities. Kleinhovia hospita Linn.(Sterculiaceae), an evergreen arbor listed in’Hainan Zhi Wu Zhi’mainly distributes in Hainan island of China. It tastes bitter, warm and toxic in nature, affecting spleen and heart meridian. The leaves and twigs of K. hospita have been used as a folkloric medicine for the treatment of pruritus, tetter, hepatitis, and scabies. In the current study, chemical constituents of C. intybus and K. hospita and their cytotoxic activities were investigated. A total of32compounds, including7new ones, were isolated and identified from the two plants by various modern chromatographic techniques and spectroscopic methods.Nine compounds were isolated from C. intybus and identified as: bacosterol-3-O-β-D-glucopyranoside (1),13,14-seco-stigma5(6),14(15)-diene-3-α-ol (2), lactucin (3),5-hydroxy-7,8,3’,4’-tetram ethoxyflavone (4),4-methoxybenzoic acid (5), threo-l-(4-methoxyphenyl)-1,2-dihydroxypropane (6),(4-methoxybenzoyl) acetic acid (7),1αr,5α-epoxy-4α-hydroxyl-4β,10β-dimethyl-7αH,10αH-guaia-11(13)-en-12-oic acid (8),1β-hydroxy-7αH,8,11βH-eudesm-3-en-8,12-olide (9). Among them, compound8was identified as a new compound and compounds1,4,9were isolated from C. intybus for the first time. Compounds2and3showed cytotoxic activity on A2780human ovarian carcinoma cell line.Twenty three compounds were isolated from K. hospita and identified as kleinhospitines A-D (la,1b,2a,2b),23R-21/23,23/27-diepoxycycloartan-1,24-diene-3,27-dione (3),21S,23R-21/23,23/27-diepoxy-21-ethoxycycloartan-1,24-diene-3,27-dione (4),21S,23R-21/23,23/27-diepoxy-21-hydroxycycloartan-1,24-diene-3,27-dione (5),21R,23R-21/23,23/27-iepoxy-21-hydroxycycloartan-1,24-diene-3,27-dione (6), gardenolic acid B (7), gardenolic-3-O-α-L-arabinopyranoside (8), gardenolic methyl acetate (9), cycloartan-1,24-diene-3,23-dione (10), taraxerone (11), taraxerol (12),2a,3β,23-trihydroxyolean-12-en-28-oic acid (13),3β,23-dihydroxy-12-oleanan-28-oic acid (14),2a,3α-dihydroxy-24-nor-4(23),12-oleanadien-28-oic acid (15), maslinic acid (16),β-sitosterol (17), daucosterol (18),22-tetraen-3-one (19), stigmasta-5,22-diene-3β,7β-diol (20),7β-hydroxysitosterol (21). Among them, compounds la, lb,2a,2b,3,8are new compounds. More interestingly, compounds la,1b,2a,2b possess a spiro-α，β-unsaturate-γ-lactamlactone side chain and compounds2a,2b are the first examples of cycloartane triterpenoids containing an a-cyclopropyl ring. In addition, compounds9,11,12,13,14,15,16,19,20,21were isolated from K. hospita for the first time. Compounds4,5,6,7,14,15,16and5,6,14,15,19,20showed cytotoxic activity on HCT116and SGC cell line respectively.In conclusion, this research has provided a solid theoretical and experimental basis for further development of C. intybus and K. hospita. |
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