Study On P2Y2and P2Y4Expression Of Spinal Cord Injured Rats

ObjectiveResearch spinal cord injury at different levels, caused on the ratneutrogena bladder. Observe urine dynamics and bladder derisory functionchanges. Explore, derisory function change and Purina P2Y2and P2Y4receptor expression about.MethodsHealthy adult SD rats50, male and female unlimited, weight180-220g.Randomly divided into3groups:10only for the control group; sacral spinalcord injured of group only20;the other for under sacral spinal cord injuredgroup. The free fall rats caused the crash spinal cord injury, form neurogenicbladder model. Weekly urine testing dynamics.30days after line drawn fromthe body depressor function test. Using immunohisto chemistry, Western blotting method P2Y2and P2Y4expression.ResultsDerisory urinary leakage in the pressure point of neural spinal cord injurythan the control group, the group of marrow nerve damage increased group isobvious; Derisory leakage point pressure in under sacral spinal cord injuredgroup control group is decreased obviously. The marrow of nerve damagegroup, derisory spontaneous excited increased frequency significantly betterthan the control group; spinal cord injury group, derisory spontaneously reducethe frequency of excitement is the control group. Purring P2Y2, P2Y4receptors in the spinal cord injury the expression of the group on a control group, under sacral spinal cord injured group is obvious; P2Y2, P2Y4militatednerve damage in spinal cord injured of reduced significantly.ConclusionsFree fall making the animal models hit method is much closer to clinicalcases in patients with spinal cord injury.Urine dynamics using the basic principle of fluid dynamics, the urinarytract dysfunction disease and normal urinary function of a science.At present, the dynamics of urine test, as an effective means of inspectionin the early diagnosis of urination dysfunction reason and choose the treatmentplan provides the basis. Spinal cord injury lead to neutrogena bladder ratsappeared bladder compliance enhance or instead performance; Depressorhyperreflexia appear or weakened. P2Y2and Y2Y4receptor expressionincreased influence bladder systolic function, appear derisory reflection abate.And the expression decrease derisory hyperreflexia.