The Significance And Expression Of NF-κB And VEGF Proteins In Cervical Squamous Cell Carcinoma

ObjectiveTo investigate the role of NF-кB, VEGF proteins in the pathogenesis andprogression of cervical carcinoma.MethodsImmunohistochemical method (P-V6000) was performed to examine theexpression of NF-кB p65and VEGF proteins in65cases of cervical squamouscell cancer,110cases of CIN and28cases of chronic cervical inflammatorytissues.Results1. Higher positive expression rate of NF-кBp65protein (70.8%) wasobserved,in the group of cervical squamous cell carcinoma, than that of in thegroups of CIN Ⅰ, CIN II and chronic cervical inflammation（P＜0.05）; but nosignificant difference statistically was observed between the groups of CIN IIIand cervical cancer（P＞0.05）;in the group of poorly differentiated squamous cell carcinoma, The positiveexpression rate of NF-кBp65protein was90.3%（28/31cases）, significantlyhigher than that of in the group of highly-moderately differentiated group(52.9%）; in the group of positive lymph node metastasis was90%（27/30cases）, significantly higher than that of in the group of no lymph nodemetastasis(54.3%）; 2. In the group of cervical squamous cell cancer, positive rate of VEGFprotein’s expression was69.2%（45/65cases）, significantly higher than that ofin the groups of chronic cervical inflammation, CIN I, CIN I（IP＜0.05）; however,no significant difference ststistically was found between the groups of CIN IIIand cervical squamous cell cancer（P＞0.05）;Higher positive expression rate of VEGF protein (83.9﹪）was found in thegroups of poorly differentiated and positive lymph node metastasis than that ofin the groups of highly-moderately differentiated and negtive lymph nodemetastasis（P＜0.05）;3. both positive expression of NF-кB p65and VEGF proteions wasobtained in38cases (58.5%) of65cases of cervical cancer; both negative theproteins in12cases（18.5%）, differentially express of the proteins in15cases(23.1%); Spearman correlation analysis showed that the expression of NF-кBp65and VEGF had a significant positive correlation (correlation coefficient, r=0.451, P <0.05).positive lymph node metastasis were found in27cases of38caseswhich are both positive expression of NF-кB p65and VEGF proteins. But only3/12cases in negative expression of both NF-кB p65and VEGFproteins（r=0.401,P＜0.05）.Conclusions1.The overexpression of NF-кB p65protein might play an important role inthe pathogenesis and malignant biologic behavious of cervical cancer.2.Both VEGF and NF-кB proteins may be collaborative in promoting thedevelopment of cervical squamous cell carcinoma.