The Protection Of Breviscapine In Cardiomyopathy Of The Diabetic Rats

ObjectiveThrough the observation of Breviscapine on cardiac structure and functionof diabetic rats,to explore the possible mechanism of Breviscapine on theprotective effect of diabetic rat hearts.Methods30healthy SD rats were randomly selected20which were injected ofstreptozotocin（STZ） by tail vein.After the modeling,20SD rats are divided intodiabetic mellitus group（DM group） and breviscapine treatment group（Bre group）randomly.10of the remaining served as normal controls（NC groups）.The Bregroup was given breviscapine injection of20mg·kg^-1·d^-1（20mg/5ml） byintraperitoneal injection,the DM group and NC group were given equivalentnormal saline by intraperitoneal injection.After12weeks,blood glucose wasmeasured by tail vein blood in each group;measure hemodynamics;calculatethe cardiac index;myocardial structural changes and interstitial collagendeposition were observe by Masson collagen staining and HE by lightmicroscopy;myocardial ultrastructure were observe by electronmicroscopy;TGF-β₁,Smad3,Smad7protein expression levels in rat myocardialof each group were detected with Immunohistochemicaltechnique;TGF-β₁,Smad3,Smad7mRNA content of each rat myocardium weredetected by RT-PCR detection.The experimental datas were processed withSPSS13.0software.ResultsCompared with NC group,blood glucose levels was increased significantlyin the DM group and Bre group （P<0.01）;Heart and left ventricular indexincreased,±dp/dtmax,left ventricular systolic pressure （LVSP） were significantlydecreased,left ventricular end diastolic pressure （LVEDP） was significantly increased（P<0.01）,increased interstitial collagen deposition（P<0.01）;proteinexpression and mRNA contents of TGF-β₁and Smad3were highersignificantly（P<0.01）,while protein expression levels and mRNA contents ofSmad7decreased（P<0.01）;Electron microscopy observe that the myocardialfilaments arranged in disorder,some dissolution and mitochondria increased,some vacuolated;Compared with the DM group,the blood glucose levels of Bregroup no significant difference in the end of the experiment（P>0.05）;cardiacindex,hemodynamics,and interstitial collagen deposition were significantlyimproved（P<0.01or P<0.05）;TGF-β₁,Smad3protein expression and mRNAlevels of Bre group in lower（P<0.01）,Smad7protein expression and mRNAlevels insignificantly increased（P<0.01）;myocardial pathological changes of Bregroup were significantly improved by light microscopy,electron microscopyobserve.ConclusionsThe Breviscapine can reduce the pathological changes of the myocardiumof diabetic rats to protect the heart function of diabetic rats,the mechanism maybe inhibit the diabetic ratsfibrosis of myocardium by regulating theTGF-β1,Smad3,Smad7protein and the mRNA expression levels of myocardialtissue to play a protective effect on diabetic rat heart.