Effects Of Tianshengyin On The Expressions Of Smad3 And Smad7 In The Kidneys Of Renovascular Hypertensive Rats

AIMS:To investigate the effect of Tianshengyin on the expressions of Smad3 and Smad7 in the kidney of renovascular hypertensive rats.METHODS:Sprague-Dawley(SD) rats with clean class were randomly subdivided into normal, sham-operated and operated group. After one week adaptability feeding, the rats were copied with two-kidney one clip(2K1C). In the operated group for renovascular hypertension, the rats underwent the operation via nipping the left renal artery. In the sham-operated group,the rats underwent the operation with only separating the left renal artery. Meanwhile there were no management on the rats in the normal group. Four weeks later, in the operated group, the rats with more than 150mmHg systolic blood pressure were randomly subdivided into model group and Tianshengyin group.The rats in Tianshengyin group were fed with Tianshengyin,While the ones in model group, sham-operated and normal group were fed with equal volume of distilled water respectively. After treated for four weeks, all rats were sacrificed. The blood pressure recorded at 9 time points respectively,i.e. the time points of pre-operation and post-operation from the first week to eighth week. Blood samples were collected for the examination of BUN and Cr with biochemistry means. The left kidneys were stained by Hematoxylin-Eosin for morphological evaluation. Fathermore, the expressions of smad3 and smad7 in the left kidneys were examined with immunohistochemical analysis.RESULTS:Compared with the sham-operated group and the normal group, the blood pressure, BUN, Cr and kidney fibrosis in model group elevated. In model group,the expression of Smad3 increased, while the expression of Smad7 decreased. There was markedly statistical difference between these groups (P<0.01). After treatment with Tianshengyin for four weeks, we found that compared with model group, BUN and Cr decreased significantly(P<0.01),although there was no significantly difference for blood pressure (P> 0.01). The degree of kidney fibrosis ameliorated in treated group, the expression of Smad3 decreased, and the expression of Smad7 increased significantly (P<0.01).CONCLUSION:These results suggest that Tianshengyin could inhibit renal fibrosis probably by means of decreasing the expression of Smad3 and increasing the expression of Smad7 in the kidneys.