The Association Study Of Neurexin3Gene Polymorphism With Delayed Encephalopathy After Acute Carbon Monoxide Poisoning

BackgroundThe delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) features a group of neuropsychiatric symptoms, such as acute dementia, extrapyramidal syndrome, and the etiology of DEACMP is still unknown. The present clinical researches of DEACMP have been at the level of cerebrospinal fluid and blood biochemistry, and the DEACMP genetic research has not been reported. The investigation on genetic susceptibility of DEACMP will help with the pathogenesis and provide beneficial evidence for its prevention and treatment.ObjectivesTo explore the association between Neurexin3(NRXN3, rs11845632, rs2196447) gene polymorphism and DEACMP, and to explore the DEACMP gene susceptibility.Methods1. The diagnostic criteria was based on the book of "Internal Medicine, sixth Edition" and Zhao Xiangzhi’s criteria on delayed encephalopathy after acute carbon monoxide poisoning (DEACMP).179DEACMP patients (104male and75female) and272patients (142male and130female) without delayed encephalopathy after carbon monoxide poisoning (ACMP) as control from Han ethnic group in Northern Henan province were recruited. The acute carbon monoxide poisoning patients were all in compliance with "National Occupational Standard for Acute Carbon Monoxide Poisoning Diagnosis GB8781-88". All patients were over40years old. 2.5ml ulnar vein blood was collected into tubes with EDTA anticoagulant respectively from fasting DECAMP patients at6:00-8:00am, and from ACMP patients within1d after they fully consciously recovered. Then the blood samples were stored at-70℃followed by DNA extraction with TIANGEN’s blood genomic DNA extraction kit.3. The NRXN3(rs1184563, rs2196447) allele frequencies were assayed with PCR-restriction fragment length polymorphism (PCR-RFLP) technology.4. All data were analyzed with SPSS17.0statistical software. The Hardy-Weinderg equilibrium compliance of genotype distribution was checked by means of goodness of fit chi-square test. The χ2test was used for association analysis.Results1. The allele frequencies of rs11845632and rs2196447in NRXN3in both DEACMP group and ACMP group were significantly different (All P values<0.05)2. When two groups were stratified by sex, for rs11845632, each genotype distribution in both female groups was significantly different (P=0.028), and the allele frequency in both female groups was not significantly different (P=0.201); but the genotype distribution and allele frequency in two male groups were not significantly different (P=0.159, P=0.795). for rs2196447, each gene distribution in both male groups was significantly different (P=0.009), and the allele frequency in two male groups was not significantly different (P=0.503); and the genotype distribution and allele frequency in both female groups were not significantly different (P=0.129, P=0.543).Conclusions1. Results indicated the association between NRXN3gene polymorphism and DEACMP, which will provide evidence for further study on DEACMP gene susceptibility.2. The risk of DEACMP in female ACMP patients would be increased for the existence of GA genotype in NRXN3gene rs11845632; GA genotype in NRXN3 rs2196447might be the DEACMP susceptibility gene in male ACMP patients, indicating that NRXN3gene polymorphism might be gender-related on the molecular genetic susceptibility of DEACMP.