Influence Of Combination Therapy With Cyclooxygenase-2Inhibitor And5A-reductase Inhibitor In The Treatment Of With Prostatic Hyperplasia Gland

Objective To discuss the effect and mechanism of combination with cyclooxygenase-2inhibitor and5a-reductase inhibitor in the treatment of rats with hyperplasia glands.Methods The forty SD male rats randomly divided into5groups by weight:Group A. normal control, Group B. BPH model,Group C.BPH model and cyclooxygenase-2inhibitor treatment; Group D.BPH model and5a-reductase inhibitor treatment, Group E. BPH model and cyclooxygenase-2and5a-reductase inhibitor treatment.28days later treatment, the prostate damp weight was measured,and the histological changes were examined by light microscopy. Detection of ki-67by IHC and TUNEL technology in prostate tissue and cells were analysed the proliferation and apoptosis index. The protein expression of cyclooxygenase-2(COX-2), the basic fibroblast growth factor (bFGF), the epidermal growth factor (EGF) and transforming growth factor-β1(TGF-β1) by IHC.Results Five groups of prostate quality index were1.24±0.04,1.90±0.07,1.73±0.08,1.79±0.07and1.66±0.06.Group B was significantly higher group A,C,D and E(P<0.00).Group E was significantly higher group C and group D (P<0.05);Obvious prostatic epithelial cells hyperplasia in group B and atrophy in group C、D and E were examined by light microscopy;Five groups of proliferation rate were0.016±0.003,0.025±0.003,0.020±0.004,0.019±0.002and0.015±0.001. Group B proliferation rate was significantly higher than those of group A,C,D and E (P<0.05).Group E proliferation rate was significantly reduced compared with group C and D (P<0.05); Five groups of apoptosis rate were0.014±0.002,0.012±0.002,0.018±0.003,0.019±0.002and0.022±0.002.Apoptosis rate of Group C,D and E were significantly higher than those of group A and B (P<0.05).Group E apoptosis rate was increased significantly than group C and D (P<0.05); The levels of COX-2、bFGF and EGF protein expression were significantly increased in group B,while TGF-β1protein expression was significantly reduced compared with those of group A, C, D and E(P<0.05).The levels of COX-2and EGF protein expression was significantly reduced in group E, while TGF-β1protein expression was significantly increased compared with those of group C and D(P<0.05).The level of bFGF protein expression was significantly reduced in group E compared with those of group C (P<0.05).There was no statistical significant of bFGF protein expression in group E and D (P>0.05).Conclusion1. Cyclocxygenase-2inhibitor can inhibit the prostate hyperplasia gland in rats.2.5a-Reductase Inhibitor Effect can inhibit the prostate hyperplasia gland in rats.3. The combination therapy with cyclooxygenase-2inhibitor and5a-reductase inhibitor than monotherapy have stronger role of inhibition on hyperplasia glands.4. Occurrence of benign prostatic hyperplasia related with such as the imbalance of inflammation factors, androgen, growth factors,and the interaction between them, and the imbalance of prostate tissue cell proliferation and apoptosis.