| Objective The detection the expressions of microRNA34a, p53and MDM2in the development process of lung cancer in rat. Explore the relationship between microRNA-34a, p53, and MDM2, and lung cancer.Methods Rats lung cancer model:Wistar rats were68,60in the left lower lobe bronchial perfusion with MCA and DEN lipiodol solution0.1ml,8perfusion0.1ml lipiodol as a control. The rats were killed at different times, made into paraffin-embedded tissue blocks, HE stain, microscopic examination and genotyped carried detection microRNA34a expression by RT-PCR method. To detected the expressions of p53and MDM2by immunohistochemical staining. Using in situ hybridization method for detection of p53and mdm2mRNA. The date used the statistical software SPSS17.0to Chi-square test and correlation statistics statistical analysis.Results1. An animal model of experimental group of48cancer in rats, induced cancer rate was80%(48/60), cancer of the large mouse in this multiple stage lesions coexist, to obtain a total of21cases of bronchial epithelial hyperplasia, atypical hyperplasia and13cases of carcinoma in situ in28cases,20cases of invasive carcinoma,16cases of metastatic carcinoma. Control group, no tumor.2. MicroRNA34a expression Use the real-time quantitative PCR method to detect microRAN34a specificity. Normal group microRNA34a expression0.825±0.093; adjacent tissues0.172±0.040; carcinoma in situ organization0.037±0.034; invasive0.019±0.005; metastatic carcinoma of0.003±0.004, there are differences between the experimental group and control group (p<0.01), carcinoma in situ group, between the invasive group and metastatic carcinoma group statistically there are significant differences(p<0.01).3. p53gene and its protein expression of p53gene expression According to the semi-quantitative product sub-standard score metastases in the control group1.04±0.49; adjacent tissues group1.96±1.01; cancer tissue,2.61±1.06; invasive group3.05±1.24; group was3.83±1.15. Analysis of variance F=30.240, P<0.05, control group and experimental group, the difference was significant (p <0.01) between groups. p53protein expression in the control group,0.79±0.48; adjacent tissues group1.92±1.04; cancer tissue,2.57±1.09; invasive group2.98±1.48; metastatic cancer group3.48±1.98, between groups analysis of variance F=21.726, p <0.05the control group with carcinoma in situ group, the group of adjacent tissues and invasive group and the metastatic carcinoma group differences were significant (p <0.01). Gene and protein expression are consistent (kappa=0.582,p=0.000).4. MDM2gene and its protein expression according to the semi-quantitative product standards MDM2gene expression score in the control group1.13±1.10; adjacent tissues group1.90±1.03; carcinoma in situ organization group,3.61±0.73; invasive group4.41±1.04; metastatic cancer group5.30±0.54. Between groups analysis of variance F=30.670, p <0.05. MDM2protein expression as a control group,1.10±1.01; adjacent tissues group1.96±1.30; cancer tissue,3.60±0.81; invasive group4.08±0.81; metastatic cancer group5.09±0.75, between groups analysis of variance F=26.072, p <0.05. Gene and protein expression are consistent (Pearson’s r=-0.681,p=0.000).5. in lung cell carcinoma, microRNAs and MDM2mRNA were both negatively correlated (Pearson’s r=-0.681, p=0.000); microRNAs and MDM2protein both showed a negative correlation (Pearson’s r=-0.671,p=0.000).; microRNAs and p53mRNA in both a negative correlation (Pearson’s r=-0.690, p=0.000); microRNAs and p53protein in both a negative correlation (Pearson’s r=-0.634, p=0.000); of MDM2and p53protein both were positively correlated(Pearson’s r=0.609, p=0.000); of MDM2mRNA and p53mRNA in both a positive correlation{Pearson’s r=0.620,p=0.000).Conclusions1. MicroRNA34a、p53and MDM2correlation with lung cancer occurrence and development. MicroRNA34a was low expression, p53and MDM2was highly expressed in lung cancer. MicroRNA34a was a negative correlation between p53and MDM2. The three were correlated with the development of lung cancer.2. MicroRNA34a、p53and MDM2were among certain internal relations, the joint detection of early diagnosis of lung cancer. |
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