The Feasibility Study About The Identification Of Fetal Cells In Cervix Mucus And Its Using In Prenatal Diagnosis

Purpose:This topic to be used fluorescent real-time quantitative PCR and polyacrylamide gel electrophoresis analysis mucous in cervical cells in total DNA STR gene loci differences, in order to achieve the appraisal female fetuses sources of cells or the purpose of genetic material.Methods:Collecting180cases of cervical mucus of6-10weeks abortion in the outpatient department of obstetrics and gynecology of Zunyi Affiliated Hospital, while extracting the villus after abortion, using the direct method to analyze the karyotype so that to determine the fetal sex. Select40cases of cervical mucus by karyotype analysis, extract DNA cell from cervical mucus, use the real-time quantitative PCR to amplify the gene D21S11, D21S1411, D21S1412, D21S1414, and amplify the length difference of the product by polyacrylamide gel electrophoresis and silver stain analysis with β-globin as positive control within.Results:in40cases of female fetuses to determine the DNA samples, specific fragments were amplified by silver-stained polyacrylamide gels and found that40specimens of27cases in the D21S11, D21S1411, D21S1412, D21S1414four loci showing positive results completely.Conclusion:1by the early stages of pregnancy, women can obtain female fetuses cervical mucus cells or genetic material, for no prenatal diagnosis and provide a new idea;2, often on the chromosomes microsatellite polymorphism sequence can be identified as one of the symbols of female fetal cells.