| Objective To observe the role of platelet rich plasma (PRP) on free fat graft’s survivalin a rat model.Methods Twenty-four rats were used in the experiment. For each rat, three groups offree fat grafts were injected into the subcutaneous layer of dorsum. Group A was givencombination of0.5ml fat and0.1ml PRP which was activated by0.02ml calcium chloride.Group B received0.5ml fat and0.1ml PRP with0.02ml normal saline. Group C was thecontrol group with0.5ml fat and0.12ml normal saline. All the fat tissue were harvestedfrom both inguinal regions. Eight rats were euthanised using pentobarbital sodium eachtime,1,2and3months after fat transplantation. Specimens were acquired and stained withhaematoxyline-eosin, and then examined the histological changes using light microscopy.The histological parameters included: adipocyte integrity, cyst formation, inflammation,fibrosis and vascular density. All the data were statistically analysed using paired t test todetermine if there were differences between the study groups. Results The platelet count showed that the platelets of PRP (4871±635.5) were muchhigher than the whole blood (617.6±61.30)(P<0.0001). Morphology observation resultsshowed that the volume of fat grafts became smaller gradually, and the graft tissue weresurrounded by a thin envelope with visible vascularity. Some of the fat grafts wereabsorbed and could not be touched2m after transplantation. Compared the volume anddiameter of the fat grafts at1m, there was no deference between the groups (p>0.05).However, the diameter of group A was significantly higher than group C (p<0.05). Onhistological examination, lager vascular density was observed in group A compared withgroup C at1m (p<0.05). And reduced inflammation reaction was also observed in group Aat3m (p<0.05). While other histological parameters were not statistically significant(p>0.05).Conclusion PRP did not enhance the survival of fat graft in the Wistar rat model. |
PDF Full Text Request