Xingnaojieyu Capsule On Stroke After Chronic Stress Depression Model Rat Hippocampal Area Synaptic Protein Effect

Objective: To study the XingNaoJieYu(XNJY) Capsule on stroke after chronicstress depression model rat hippocampal area SYT and SYTmRNA in thehippocampal region of the distribution and expression of effect. To explorethe treatment of post-stroke depression mechanism of post-strokedepression,In order to provide an advanced and effective treatment method.Methods：To use the left middle cerebral artery occlusion (MCAO) combined withchronic unpredictable mild stimulation(CUMS)and solitary curing methodcomposite molding method causes the PSD rat model.selection of Sprague-Dawley(SD) and half of the rats, according to the field experiment score, the ratswere randomly divided into6groups: A：model group；B：blank control group；C：Prozac group；D：XNJY capsule high-dose group；E:XNJYJieyu capsule dosegroup and low dose-group F.Except the blank group and other groups all usethe composite molding method to establish PSD model.After the model wasestablished,A、B groups given the saline lavage，C groups were given byBaiyoujie capsule，D、E、F groups respectively according to the high，mediumand small doses of XNJY capsule lavage.Each groups lavage method continuouslyfor29days and cut off the head remove brain,anatomy and complete strippingof the hippocampus,rats were observed Open-Field experiments and sucroseconsumption behavior changes，application of immunohistochemistry observa-tion of brain hippocampus histological pathological tissue sections, usingreal-time fluorescence quantitative RT-PCR assay for detection of rat hipp-ocampal SYTmRNA levels，statistical analysis of experimental data,discuss thecompound preparation of traditional Chinese medicine XNJY Capsule on patientswith post-stroke depression rats hippocampal SYT effects, and to discuss itsmechanism of action. Results:through CUMS combine MACO method and solitary compound model methodwas successfully preparation SD rats PSD model, rats apparent behavior change,and brain morphology structure change, explain this method can reproduce theideal rats PSD model.1XNJY Capsule on PSD behavioral changes in rats.After Manufacturing Rat model，The rats in the model group weight increasesslowly，Open-Field experiment in vertical movement, horizontal movementscored significantly prolonged, sugar consumption decreased significantly，compared with the normal group, with significant difference(P＜0.05),thisresults suggest that post-stroke depression model can affect rat body weightand behavior index；After the treatment，compared with model group，Each XNJYcapsule group and Prozac group behavior various index were obviously improved，all can obviously increase the PSD rats weight，has the significant diffe-rence(P＜0.05)，XNJY capsules, high-dose and doses group and Prozac efficacywithout significant difference(P＞0.05)；Point out both of them can improvePSD rat body weight and behavior indexes.2XNJY capsule on PSD model rat hippocampal SYT immunohistochemistry methodsresultsImmunohistochemical results show that compared with the blank group,model group of big rat hippocampal SYT area of positive express lighter color,its average light density values decrease, a statistically significant (P＜0.01). Compared with model group, XNJY capsules each dose group and the rathippocampal Baiyoujie group SYT express a model group area enhanced obviously,average light density values enhanced markedly,a statistically significant(P＜0.05or P＜0.01), but the group compared with blank, positive cell countand light density values are still down and reduce (P＜0.05).3XNJY capsule on PSD model rat hippocampal SYTmRNA influenceCompared with blank Group，model group the hippocampus SYTmRNA expressdeclined obviously, and has significant difference (P＜0.05),compared withmodel group, XNJY Capsule, and the dose group of rat hippocampal SYTmRNA Prozac group express increased significantly, has the remarkable differences(P＜0.05)Conclusions:PSD rats appear lost appetite，decreased activity ability，lossof interest and anhedonia are similar with the feature of human depression.After filling the stomachtreatment，Antideperssant treatment can obviousimprove behavioral changes.These showed that copying dperession model wassueeessed. Through the up-regulation of SYTmRNA expression in hippocampus,enhanced synaptic plasticity exert antidepressant effects may be one of themechanisms of XNJY for PSD.