| Background and Objective: Prostate carcinoma is the most common urinary tract tumor in western countries and its incidence in China also shows a rising trend year by year. Surgery or hormone therapy is the most effective treatment measures, but the measures are easy to recurrence and transfer. Prostate carcinoma seriously affects the urinary system cancer prognosis survival. The pathogenesis of PCa has not yet been clarified, and the level of regulation by multiple genes may be involved in many important aspects of the disease. This article was to investigate the expression and Significance of Skp2、P27and PCNA protein in Prostate carcinoma tissues and relationship between Skp2、P27and PCNA in the development of Prostate carcinoma.Materials and Method:We collected57cases of prostate cancer and25cases of benign prostatic hyperplasia which were all confirmed by pathology specimens from prostate biopsy or surgery and from second hospital of Tianjin medical university in August2007-August2010. PCa patients’average age is65with a range of48-82years. According to the level of PSA of PCa,57cases of prostate cancer can be divided into three groupes:<4ug/L28cases,4～10ug/L16cases,>10ug/L13cases points). According to the2002American Association of Cancer(AJCC) TNM staging system in57patients with PCa were divided into two groups:19cases of T1-T2and38cases of T3-T4. BPH patients aged60-78years and meaned69years. All the patients were not received preoperative radiotherapy, chemotherapy or anti-androgen therapy. Staining steps were carried out according to kit instructions, and we choose positive prostate cancer histological section as positive control, with PBS replaced the antibody as negative control. The result were observed by light microscope, and buffy particles in cells were considered as positive cases. SPSS19.0software application was used for all analysis and statistical significance was defined as P values less than0.05.Results:(1) In57cases of prostate cancer,33patients showed positive expression of SKP2, the positive rate was57.89%; In the25cases of benign prostatic hyperplasia tissues, the expression were5cases, the positive rate was20.00%. Two groups were compared. The difference of prostate cancer and benign prostatic hyperplasia group was statistically significant(P<0.01). The positive expression rate of Skp2in prostate cancer was markedly higher than that benign prostatic hyperplasia group20%(P=0.028). The Skp2correlated positively with the level of PSA (P=0.000), extraprostatic extension(P=0.033), clinical stage(P=0.001).(2) In57cases of prostate cancer,21patients showed positive expression of P27, the positive rate was36.84%; In the25cases of benign prostatic hyperplasia tissues, the expression were23cases, the positive rate was92.00%. Two groups were compared. The difference of prostate cancer and benign prostatic hyperplasia group was statistically significant(P<0.01). The positive expression rate of P27in prostate cancer was markedly lower than that benign prostatic hyperplasia group. The P27correlated negativly with the level of PSA (P=0.001), extraprostatic extension(P=0.000), clinical stage(P=0.001).(3) In57cases of prostate cancer,39patients showed positive expression of PCNA, the positive rate was68.42%; In the25cases of benign prostatic hyperplasia tissues, the expression were8cases, the positive rate was32.00%. Two groups were compared. The difference of prostate cancer and benign prostatic hyperplasia group was statistically significant(P<0.01). The positive expression rate of P27in prostate cancer was markedly higher than that benign prostatic hyperplasia group. The PCNA correlated positively with the level of PSA (P=0.000), extraprostatic extension(P=0.006), clinical stage(P=0.003).(4) Expression of P27was negatively correlated with expression of Skp2and P27(r=-0.640、-0.728,P<0.001).Expression of Skp2was positively correlated with expression of PCNA(r=0.814,P<0.01).Conclusion:(1) This study suggests that positive expression rate of Skp2and PCNA showed an increasing trend in BPH and PCa group, and P27showed a decreasing trend,but the differences were only statistically significant between the BPH and PCa group.(2) The expression of Skp2、P27andPCNA in prostate cancer was matter with the level of PSA, extraprostatic extension, clinical stage.But there was no relationship with the age, lymph node metastasis and distal metastasis.(3) Skp2may have cooperation with P27and PCNA in the process of molecular regulation of PCa via synergistic actions, promoting the occurrence and development of prostate cancer.(4) The combined detection of Skp2,P27and PCNA proteins can be used to evaluate the incidence of prostate carcinoma development, prognosis, and provide an important basis for drug treatment. |
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