Effect Of Uygur Medicine Maizhuni On The Expression Of MMP-1,MMP-3, MMP-13,TIMP-1,IL-1β And Aggrecan In Rat Osteoarthritis Models

Objective: To establishe the OA model, To investigate the expression of matrixmetalloproteinase1,3,13,Interleukin-1βand tissue inhibitor of matrix metalloproteinase1inarticular cartilage and the intervention effects of Uygur medicine Maizhuni.Methods: Atotal of40healthy male SD rats were randomly divided into Maizhuni group and， modelgroup，shamed oPeration group and normal control grouP. Rats were treated with Uygurmedicine Maizhuni or saline by gavage in the2nd week after model construction for4weeks.Results: The modelof of OA animal model was Successfully established.ComPared with model group, the degree of rat knee joint cartilage degeneration inMaizhuni group was alleviated, The cartilage general score and Mankin score in MaizhunigrouP was lower than that in the model group (P <0.05), The score of Maizhuni groupwas higher than in the shamed operation group and normal control group(P <0.05)，thereare no differece beteeen normal control group and shamed oPeration group(P>0.05),Theexpression of matrix metalloproteinase1,3,13in knee joint chondrocytes of Maizhunigroup decreased than that of the control group(P <0.05)；The expression of interleukin1βin knee joint chondrocytes of Maizhuni group also decreased than that of the controlgroup(P <0.05);The expression of Aggrecan in knee joint chondrocytes of Maizhunigroup increased than that of the control group;The expression of tissue inhibitor of matrixmetalloproteinase1in Maizhuni group increased than that in the model group (P <0.05).Conclusion: These findings indicate that Uyghur medicine Maizhuni may protect articularcartilage by down-regulating the expression of matrix metalloProteinase1, up-regulatingthe expression of tissue inhibitor of matrix metalloproteinase1,Reduce the expression ofinterleukin-1β.