Experimental Study In Effects Of Xu-zao-geng-ping Powder On The Histomorphology Change Of Arteries And MMP-9, TIMP-1in Climacteric Hypertension Rats

Objective: Through study of the effects of Xu-zao-geng-ping Powder in climacterichypertension rats on histomorphology change of vascular and MMP-9，TIMP-1researchto explore reverses vascular remodeling and possible mechanism in Climacterichypertension rats. Methods: Surgical remove ovaries of SHR rats to establish climacterichypertension model and evaluate it. Randomly divided SHR rats into climacterichypertension traditional Chinese medicine, climacteric hypertension western medicine,climacteric hypertension, fake operation and SHR groups. Set Wistar group as a normalcontrol group. Drench Xu-zao-geng-ping powder to climacteric hypertension traditionalChinese medicine group and Valsartan capsule to climacteric hypertension westernmedicine. The other groups were drenched saline solution. Observation of morphologicalchanges in vascular tissues of rats in each groups, measuring MT, LD, MT/LD,monitoring changes of blood pressure in groups of rats and comparing MMP-9, TIMP-1level in serum of different groups. Results:1. Vaginal smear of model rats showed norenew of estrous cycle change. Rat serum E2, T, P levels were significantlyreduced(P<0.05); FSH and LH level were significantly increased (P<0.05).2. Comparedwith the climacteric hypertension group, fake operation group and SHR group, bloodpressure of climacteric hypertension traditional Chinese medicine and climacterichypertension western medicine group in rats dropped significantly before and afterdrenching drug.3. Morphological changes was significant in vascular tissues ofclimacteric hypertension rats. Their LD reduce than other groups, MT, MT/LDsignificantly increased (P<0.05). LD of fake operation, SHR group decreased thanclimacteric hypertension traditional Chinese medicine, climacteric hypertension westernmedicine and Wistar group, but MT, MT/LD of fake operation increased (P<0.05). LD of climacteric hypertension traditional Chinese medicine and climacteric hypertensionwestern medicine groups decreased than Wistar group, but their MT, MT/LD increased(P<0.05).4. The serum levels of MMP-9, TIMP-1of climacteric hypertension groupsignificantly increased than the other groups (P<0.05). Compared with the climacterichypertension, fake operation and SHR groups, MMP-9levels in serum of climacterichypertension traditional Chinese medicine and climacteric hypertension western medicinegroups decreased, but TIMP-1levels increased (P<0.05). Conclusion:1. Establishedclimacteric hypertension rat model by removal of ovaries in SHR rats.2. Vascular tissuepathological changes can be increased in climacteric hypertension rats.3. MMP-9levelsof serum increased to stimulate TIMP-1level of serum compensatory increased. Disorderof MMP-9and TIMP-1broke the dynamic balance result the vascular remodeling.4.Findings of this study indicate that the "Xu-zao-geng-ping Powder” can reduce bloodpressure in climacteric hypertension rats significantly and reduce vascular tissue damagecaused by reducing MMP-9and increasing TIMP-1in serum to restore ECM controlbalance. It probably is the effective mechanism for preventing and reversing vascularremodeling in climacteric hypertension rats.