The Ampelopsis Vitro Anti-tumor Active Ingredient Screening And The Mechanism Of Study

Ampelopsis japonica(Thunb.)Makino is a plant,.Its dried roots have been used as traditional medicine for the treatment of anodyne and febrifuge. and only contained in the "Shen Nong’s Herbal Classic". Bitter, acrid, slightly cold, clearing and detoxifying, carbuncle Sanjie, grabbing a sore myogenic effects for the treatment of the ulcer back, boils, scrofula, burns embolism. Modern pharmacological studies have shown that the drug has antibacterial, immunomodulatory, inhibit hair growth and anti-tumor effect. Clinical applications and pharmacological studies on Ampelopsis focus is more than an antibacterial effect, while its anti-tumor activity has been gradually worthy of our attention. In this study, the biological activity under the guidance of the active ingredient separation, screening out the Ampelopsis antitumor activity of parts of its active ingredient, and to evaluate its antitumor mechanism of action.,for the Ampelopsis further research and development to provide important experimental data. In this paper, the following:1MTT assay was used to select the human hepatoma cell HepG2, dialogue modulation of the alcohol extract and ethanol extract of petroleum ether, ethyl ether, ethyl acetate, butanol, aqueous solution of parts of the anti-tumor activity. The results showed that the two parts of ether parts of ethyl acetate and the best part of the anti-tumor activity of the Ampelopsis.2By silica gel column chromatography, isolated from the ether parts of ethyl acetate five parts were petroleum ether, ethyl ether, ethyl acetate, ethanol, methanol part. MTT assay was used the5parts of the anti-tumor activity screening. The results showed that the best part of ether and ethyl acetate part of the anti-tumor activity of the Ampelopsis two parts.3By silica gel column chromatography, LH-20Sephadex column, high performance liquid chromatography separation means separation as a guide to the biological activity, from the best activity of the ether part of the compounds were isolated and characterized by IR1H-NMR,13C-NMR, and other spectral methods, structural analysis of isolated monomer compounds ultimately determine them for:beta-Valley steroid alcohols、oleanolic Acid、 gallic acid、palmitic acid、resveratrol.4MTT assay was used, the alignment oleanolic acid、gallic acid、 resveratrol, anti-tumor activity, the results show that the compounds did not gallic acid, the inhibitory effect of oleanolic acid on human hepatoma cell line HepG2is the most obvious. Gallic acid with an IC50of48hours was18.3mg of L-1, and48hours of oleanolic acid IC50value of38.4mg·L-1. Therefore, the main active ingredient of Chinese medicine Ampelopsis from the anti-tumor effect of gallic acid.5Studies explore the Ampelopsis, gallic acid, anti-tumor role of mechanisms, the results show, gallic acid in vitro can significantly with the inhibition of tumor cell HepG2the growth and proliferation and induced tumor cell differentiation, and inhibit the expression of tumor cell telomerase activity in the induction of apoptosisapoptosis and necrosis together.6Acute toxicity test found that the mentioned components of Ampelopsis water, alcohol extraction component, ethyl acetate mentioned components and components not measured the LD50of; the maximum tolerated dose trial, measured four components of the MTD values120,154.2,696.6and3.75g·kg-1·d-1(crude drug), respectively, equivalent to the clinical70kg adult per kilogram of body weight daily dose of930.9times, times1194.7,5398.6times,29times, showing Ampelopsis has better security.