The Effcts Of Propofol On NF-KB Activation And HIF-1a MRNA Expression Of Human Umbilical Vein Endothelial Cells Sufferring From Intermittent Hypoxia

Background:Intermitttent hypoxia leading to oxidative stress is the main reason for heart cerebrovascular disease that is the important complication of Obsructive Sleep Apneahypopnea Syndrome (OSAHS). Propofol a fast action、strong force、shot half-time intravenous anesthesia drugs can restrain the production of ROX and eliminate ROX.Some reseach show that Propofol can protect viscera from ischemia reperfusion injury during the operation.Meanwhile,along with density increase the ability of straining inflammatory response is larger.However,at home and abroad the study on propofol defending cells sufferring from intermittent hypoxia is less.Now most reseachers think the pathological physiology of ischemia reperfusion is similar with the intermittent hypoxia’s.So we speculate propofol can also protect the endothelial cell in the intermittent hypoxia environment.and there is different effect because of different concentration.Objective:To observe the impact of different concentration propofol on the gene expression of hypoxia-inducible factor-1(HIF-1) and protein expression of nuclear transcription factor kB(NF-KB) of the human umbilical Vein Endothelial Cells(eahy926) in the intermittent hypoxia environment. Method:To establish intermittent hypoxia model of eahy926lines. The cells were randomly divided into the control group and experimental group. The cells of control groups marked Nlgroup(propofol concentration Oug/ml) N2group(propofol concentration3ug/ml) N3group(propofol concentration6ug/ml) were cultured in the room air. The cells of experimental groups marked T1group(propofol concentration Oug/ml) T2group(propofol concentration3ug/ml) T3group(propofol concentration6ug/ml).were cultured in the intermittent hypoxia environment. The activation of the nuclear NF-KB were detected by western blot. The gene expression of HIF-1a were detected by Real-time PCRResults:1. western blot:Compared with the N1group, the quantity of the nuclear NF-KB in the T1group obviously increase (p<0.01). The quantity of the nuclear NF-KB in the T2and T3groups decreased than in the T1group (p<0.01). The quantity of the nuclear NF-KB in each control group shows no significant different.2. Real-time PCR:Compared with the control group Nl, the gene expressions of HIF-la obviously increased in T1group(p<0.01). Compared with the T1group, the gene expressions of HIF-la in the T2and T3groups significantly decreased (p<0.01). The gene expressions of HIF-la in each control group shows no significant different.Conclusion:The intermittent hypoxia can active the NF-KB and induce the expression of HIF-la mRNA of Eahy926cell lines. Propofol inhibited activation of NF-KB and the expression of HIF-la mRNA of Eahy926cell lines in the intermittent hypoxia environment. At the same time, the inhibition effect of propofol show concentration dependent.