| Root canal therapy is the most perfect and effective treatment for pulpal and periapical diseases. As one of the important steps of root canal therapy, irrigating the canal can eliminate bacteria and inhibit its infectious metabolites there. At present,3%H2O2and0.5%～5.25%NaCLO are commonly used as root canal irrigating drugs in clinic practice. Apparently, these drugs are very antibacterial, but they also have strong cytotoxicity, which can easily irritate the periapical tissue to damage.3%H2O2has many effects, such as oxidation, deodorization and sterilization. Nevertheless, in the process of root canal irrigation, mishandling can also cause pain, even emphysema. Sodium hypochlorite solution has a strong effect of histolysis. When the apical foramen is destroyed or not fully closed, sodium hypochlorite solution can overflow it, damage periapical tissue and affect the healing of periapical lesion. Traditional Chinese medicine has a long history in our country, it has a wide range of application due to the rich resources and low costs. Traditional Chinese medicine irrigations contain a variety of ingredients. Because of their wide range of antimicrobial spectrum, great anti-bacterial properties and few side effects, scholars have paid more and more attention to them. Chinese Gall contains a large amount of tannic acid, which has a strong antibacterial property, especially on actinomyces naeslundii and staphylococcus aureus. What’s more, Chinese Gall also has a good astringent action. Baical skullcap root has a variety of pharmacological effects, such as anti-inflammatory action, antioxidation and removing free radicals. Baical Skullcap Root also has a good inhibitory effect on Porphyromonas gingivalis, especially its endotoxin. In recent years, Chinese Gall and Baical Skullcap Root have separately been widely used in various areas of oral medicine, and certain results are achieved. However, research of compound preparation in dental fields, especially as root canal irrigating drugs, are seldom reported at home and abroad.Purpose:1. To triturate Chinese Gall and Baical Skullcap Root extract, which is the compound preparation extracted from the above two kinds of traditional Chinese medicine as a new endodontic irrigation.2. To study the cytotoxicity of the Chinese Gall and Baical Skullcap Root extract in periapical tissue and whether it is reversible, then comparing the toxicity with that of0.5%sodium hypochlorite solution. We hope that the study can provide the experimental basis for the clinical selection of ideal endodontic irrigation.Method1.Chinese Gall extract and Baical Skullcap Root extract were mixed up in equal proportion, then configured into different solutions by DMEM medium containing10%FBS and1%Benzylpenicillin、Streptomycin, with final concentration of5μg/ml、25μg/ml、50μg/ml、75μg/ml、100μg/ml.2. The human periodontal ligament fibroblasts were cultured in vitro to4-6generations, then inoculated on96-well plate and divided into three groups:the blank control group, the experimental group and the positive control group. DMEM culture medium containing10%FBS and1%Benzylpenicillin Streptomycin, Chinese Gall and Baical Skullcap Root extract with five different concentrations and0.5%sodium hypochlorite solution were respectively added into groups above. At last, the cell proliferation activity and degree of recovery in each group are tested by MTT method.Result:1.Chinese Gall and Baical Skullcap Root extract with concentrations of5μg/ml～50μg/ml can promote the multiplication of HPLFs significantly, and the extract with concentration of25μg/ml can attain the maximum effect, and there was significant difference (P<0.05) when compared to the control group. In the group of100μg/ml, the extract had an apparent inhibition to the multiplication of HPLFs, and there was significant difference (P<0.05) when compared to the control group.0.5%sodium hypochlorite solution, an endodontic irrigation that was commonly-used in clinical, had an obviously toxic effect on HPLFs, which was significantly different from the other groups (P<0.01).2. HPLFs were cultivated24h in Chinese Gall and Baical Skullcap Root extract with the concentration of100μg/ml and0.5%sodium hypochlorite solution, after that we removed the drugs and continued to cultivate HPLFs72h. We found that there was no significant difference(P>0.05) between the control group and the group that was cultured in Chinese Gall and Baical Skullcap Root extract with the concentration of100μg/ml, while0.5%sodium hypochlorite solution inhibited the proliferation of HPLFs significantly comparing with the other groups(P<0.01).Conclusion:Chinese Gall and Baical Skullcap Root extracts with the concentrations of5μg/ml～50μg/ml have no cytotoxicity, and they can promote the proliferation of periodontal ligament fibroblast. Chinese Gall and Baical Skullcap Root extract with the concentration of100μg/ml has slight but reversible cytotoxicity, while0.5% sodium hypochlorite solution has strong, irreversible cytotoxicity.All in all, as for its toxic effect, the Chinese Gall and Baical Skullcap Root extract is weaker than that of the0.5%sodium hypochlorite solution. The study provided a good beginning for that Chinese Gall and Baical Skullcap Root extract will act as the ideal endodontic irrigating drugs for the further study. |
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