Studies On The Antagonistic Effect Of Curcumin And Selenium On Formaldehyde-induced Toxicity

Objective:Formaldehyde is a kind of confirmed human carcinogen. With a wide applicationof formaldehyde, toxic and pollution for a long time, it becomes one of the main pollutantsof indoor air which damages to human being are bigger. As a kind of natural antioxidant,curcumin has lots of pharmacological activities. Selenium is an essential trace element ofthe humen. Existing studies have shown that selenium can be used in cancerchemoprevention, has antioxidant and anti-mutagenic effect. In addition, selenium alsoalleviate a lot of heavy metal poisoning, and has antagonistic relationship for someelements.By studying the antagonism role of curcumin and selenium on the toxicity offormaldehyde, it can provide a new method to study the way of suppressing or weakeningthe health hazards of formaldehyde.Methods: Using A549cells on the logarithmic growth phase which growing in goodcondition doing experiments. Experimental groups for normal controls,0.1mmol/Lformaldehyde exposure group, curcumin and selenium antagonist formaldehydegroups.Make comparisons between groups to test if AP-1and NF-κ B expression, theDPC rate, total superoxide dismutase (SOD) vigor, total nitric oxide synthase (NOS) vigor,glutathione peroxides (GSH–PX) vigor, malondialdehyde (MDA) content in A549cellshave statistical significance.Results:1.the antagonistic effect of curcumin on formaldehyde1.1Antagonitic effect of curcumin on formaldehyde-induced oxidative damage in A549cellCompared with control group,formaldehyde can make the total SOD activity in A549cells,total NOS activity and GSH-Px activity decreased (all P<0.05), increased release ofMDA(P<0.05).Compared with the formaldehyde exposure group,2.5mg/L-20mg/Lcurcumin group increased the GSH-Px activity(P<0.05),2.5mg/L-40mg/L curcumindecreased MDA content(P<0.05). Compared with control group,10mg/L，20mg/L curcumingroup had no statistically significant（P>0.05）.1.2Antagonitic effect of curcumin on formaldehyde-induced DPC damage in A549cell0.1mmol/L Formaldehyde can produce significant effects of DPC(P<0.05),Comparedwith control0.1mmol/L Formaldehyde exposure group, curcumin can make the DPC ratedown(P<0.05).1.3Effect of curcumin on formaldehyde induced AP-1and NF-B expression in A549cellFormaldehyde would be damage A549cells,NF-κB is activated（P<0.05）,compared with the formaldehyde exposure group,curcumin antagonist group decreased NF-κBactivity(P<0.05),But2.5mg/L,5mg/L curcumin group NF-κB activity was higher thanthat of the control group（allP<0.05）,Compared with control group,10mg/L，20mg/Lcurcumin group had no statistically significant.Formaldehyde can increase the activity ofAP-1（P<0.05）,compared with the formaldehyde exposure group,curcumin antagonist groupdecreased AP-1activity(P<0.05),Compared with control group,5mg/L，10mg/L，20mg/Lcurcumin group had no statistically significant(P<0.05).2.the antagonistic effect of selenium on formaldehyde2.1Antagonitic effect of selenium on formaldehyde-induced oxidative damage in A549cellCompared with control group,formaldehyde can make the total SOD activity in A549cells,total NOS activity and GSH-Px activity decreased (all P<0.05), increased release ofMDA(P<0.05).Compared with the formaldehyde exposure group,1.25mg/L-10mg/Lselenium group increased the GSH-Px activity(P<0.05),2.5mg/L-40mg/L seleniumdecreased MDA content(P<0.05). Compared with control group,10mg/L，10mg/L seleniumgroup had no statistically significant（P>0.05）.2.2Antagonitic effect of selenium on formaldehyde-induced DPC damage in A549cell0.1mmol/L Formaldehyde can produce significant effects of DPC(P<0.05),Comparedwith control0.1mmol/L Formaldehyde exposure group, selenium can make the DPC ratedown(P<0.05).2.3Effect of selenium on formaldehyde induced AP-1and NF-B expression in A549cellFormaldehyde would be damage A549cells,NF-κB is activated（P<0.05）,comparedwith the formaldehyde exposure group,selenium antagonist group decreased NF-κBactivity(P<0.05),But1.25mg/L,2.5mg/L selenium group NF-κB activity was higher thanthat of the control group（allP<0.05）,Compared with control group,5.0mg/L，10.0mg/Lselenium group had no statistically significant.Formaldehyde can increase the activity ofAP-1（P<0.05）,compared with the formaldehyde exposure group,selenium antagonist groupdecreased AP-1activity(P<0.05),Compared with control group,10mg/L selenium grouphad no statistically significant(P<0.05).Conclusion:1.Formaldehyde can be produce oxidative damage on A549cells,make the total SODactivity,total NOS activity and GSH-Px activity decreased,increased release ofMDA.Curcumin can increase the total SOD activity,total NOS activity and GSH-Pxactivity,decreased release of MDA.With the increase of curcumin concentration,formaldehyde-induced oxidative damage in A549cell antioxidant enzymeactivity is gradually restored to its original level,the antagonism of formaldehyde inducedoxidative damage of capacity increasingFormaldehyde can significantly induce the formation of DPC, curcumin canantagonize the formaldehyde-induced the DPC.Formaldehyde can damage A549cells,Formaldehyde can increase the activity of AP-1and NF-κB,curcumin in certain concentration can antagonize the AP-1and NF-κB activationexpression,In order to reduce its carcinogenic effect.2.Formaldehyde can be produce oxidative damage on A549cells,make the total SODactivity,total NOS activity and GSH-Px activity decreased,increased release ofMDA.Selenium can increase the total SOD activity,total NOS activity and GSH-Pxactivity,decreased release of MDA.With the increase of seleniumconcentration,formaldehyde-induced oxidative damage in A549cell antioxidant enzymeactivity is gradually restored to its original level,the antagonism of formaldehyde inducedoxidative damage of capacity increasingFormaldehyde can significantly induce the formation of DPC, selenium can antagonizethe formaldehyde-induced the DPC.Selenium in certain concentration can antagonize the AP-1and NF-κB activationexpression,In order to reduce its carcinogenic effect.