Cytotoxic Effection In Raw264.7Induced By Uranium Ore Dust And Expression Of TGF-β1

Objective: To explor the cytotoxicity of pulmonary macrophage and expression of TGFbeta1induced by uranium ore dust.Methods: RAW264.7cells were cultured in vitro. The experiment was respectivelyprovided experimental group with uranium ore dust and control group with Serum-freemedium. The experimental group was treated with uranium ore dust suspension(120μg/ml) for2、4、8、16、24、32hours, and RAW264.7cell proliferative activity wasobserved by MTT assay; Cell morphology was observed by HE staining method; Cellapoptosis was observed by acridine orange staining; The levels of·OH、H2O2and MDAwas detected by colorimetric method; The expression of TGF beta1protein wasdetected by ELISA.Results: RAW264.7cells were treated with uranium ore dust suspension (120μg/ml)for2、4、8、16、24、32hours, MTT assay results showed that RAW264.7cellsproliferation activity significantly reduced (compared with the control group, P<0.05),and with the extension of time of treating with uranium ore dust suspension, RAW264.7cells proliferation activity increasingly lower; HE staining showed uranium dust groupshuttle or irregular cell increased, the number of multinucleated cell significantincreased, cytoplasm lost, nuclei exposed and cell morphology was poor, and with theextension of time, the appearance of cell morphology was getting worse; By acridineorange staining, red or orange apoptotic/necrotic cells increased significantly, and thenumber of necrotic and apoptotic cells gradually increased with the time extension oftreating with uranium ore dust suspension; The levels of OH、H2O2、and MDAof cellculture supernatant in uranium dust groups significantly increased (compared with thecontrol group, P<0.05),and with the extension of time, the levels continued to rise; The experimental results of ELISA showed that uranium ore dust could cause TGF-β1expression of RAW264.7cells culture supernatant increased (compared with controlgroup, P<0.05), and with the extension of time, TGF-β1levels was gradually increasedto peak at24hours.Conclusions:1. The uranium ore dust can cause apoptosis or disintegration of RAW264.7cells, andinhibit cell proliferation activity.2. Uranium dust can cause cell to the culture supernatant increased free radical levelsand induce lipid peroxidation.3. Uranium dust can induce macrophages to secrete TGF-β1, causing the levels ofTGF-β1in cell culture supernatant increased.