The Change Of NSE And TNF-α In Cerebral Ischemic Rats Treated By Polyene Phosphatidylcholine Injection

Objective:The purpose of this study were to evaluate the changes of NSE and TNF-αlevels in serum and the side of ischemic brain in rats with focal cerebral ischemia atdifferent times, and try to offer a new therapeutic strategy for brain ischemia.Methods:The focal cerebral ischemia animal modles were according to the methods ofLonga’s.The eighteen male SD rats of thirty with neurological deficit graded2wererandomly devided into3groups (n=6):operated group,early-stage administered groupand lated-stage administered group.In addition,we choose six as sham group.The twogroups with drug were intraperitoneal injected into the polyene phosphatidylcholineinjection after the two hours or on the third day of operation at a dosage of80mgevery kilogram (80mg/kg) continuous three days, then the same amout of the salinewere injected into the four groups when they did not inject drug. Neurological scoreswere performed on the6thmorning of operation, and the content of NSE and TNF-α inserum were measured by enzyme-linkd immunosorbant assay(ELASA).Theexpression of NSE and TNF-α protein in the side of ischemic brain were detected byWestern blot analysis.Results:(1)Compared with neurological scores at the end of study, the sham group andadministered group were lower than operated group(P<0.05).(2) Compared with thecontent of NSE and TNF-α, operated group was higher than the shamgroup(P<0.05).The two groups with drug were respectively lower than the operatedgroup (P<0.05),but the two with drug had no statistically difficulties between each other (P>0.05).(3) Compared with the expression of NSE protein in the side ofischemic brain, the operated group was remarkable higher than shamgroup(P<0.05).The two groups with drug were respectively lower than the operatedgroup(P<0.05),but there were no statistically difficulties between each other (P>0.05).(4) Compared with the expression of TNF-α protein in the side of the ischemic brain,operated group was higher than sham group(P<0.05).The two groups with drug wererespectively lower than the operated group(P<0.05),and the lated-stage administeredgroup was significantly lower than early-stage administered group (P<0.05).Conclusion:1. Polyene phosphatidylcholine injection can not only reduce the content of NSEand TNF-α in serum but also in ischemic brain in rats with focal cerebral ischemia;2. Polyene phosphatidylcholine injection may be can significantly decreaseTNF-α after the3thday;3. Polyene phosphatidylcholine injection may be can play a neuro-protectiveeffect through decrease TNF-α in rats with focal cerebral ischemia.