The Expressions And Clinical Significances Of Magl In Human Primary Hepatocellular Carcinoma

Objective:To discuss the expression and clinical significance of Monoacylglycerol lipase (MAGL) in primary hepatocellular carcinoma(HCC) tissues.Methods:To compare the differences of expressions of MAGL mRNA and protein in normal liver tissues, adjacent nontumoral liver tissues, highly differentiated HCC tissues and poorly differentiated HCC tissues by the application of real-time PCR, immunohistochemical method and Western blot.Results:(1) The results of immunohistochemical test shows that MAGL protein mainly expressed in nucleus of HCC tissues, while mainly in cytoplasm of normal liver tissues and adjacent nontumoral liver tissues. The IOD values in Normal liver tissues, adjacent nontumoral liver tissues and highly differentiated HCC tissues and poorly differentiated HCC tissues were respectively6742±974、12473±1168、27877±1083,36479±964. Expression of MAGL protein showed significant difference in the four groups(P<0.05). (2) The results of real-time PCR showed that the expression of MAGL mRNA in adjacent nontumoral liver tissues, highly differentiated HCC tissues and poorly differentiated HCC tissues were respectively2.4264±0.3962,4.1597±0.5355and7.3309±0.6505, in which showed significant difference(P<0.05).(3) The results of Western blot showed that MAGL protein expressed weakly in normal liver tissues and adjacent nontumoral liver tissues, strongly positive in HCC tissues. In normal liver tissues, adjacent nontumoral liver tissues, highly differentiated HCC tissues, poorly differentiated HCC tissues, the expression of MAGL protein were respectively0.504±0.0228、0.5605±0.0210、0.6892±0.0257and0.8134±0.0237. Expression of MAGL protein showed significant difference in the four groups (P<0.05)Conclusion:The highly expression of MAGL is positively related to the malignancy phenotype of primary hepatocellular carcinoma, which probably plays an important role in invasions and metastasis of cancer cells in vivo.