The Relationship Between Dendritic Cells In Prostate Carcinoma Microenvironment And Various Types Of Blood Cells And Its Clinical Prognostic Value Evaluation

Background：Dendritic cell vaccine has been widely used in tumor cellsof the immune therapy. Immune therapy that kills tumor cells and does notdamage normal cells has a broad application prospect. That use of vaccinecarrier targeted activate dendritic cells in the clinical trials made certainprogress. The mammalian blood system contains more than ten distinctmature cell types including red blood cells (erythrocyte),megakaryocytes/platelets, myeloid cells (monocyte/macrophage andgranulocytes), mast cells, T-and B-lymphocytes, natural killer (NK) cellsand dendritic cells (DCs). The concept such diverse cell types are allderived from a common progenitor cell. So there may be some correlationbetween the quantity of mature blood cells in the blood system and thequantity of dendritic cells. This research emphasized on the correlationbetween the quantity of various dendritic cells in prostate carcinomamicroenvironment and various types blood cells. It is hoped to study the features of dendritic cell development in prostate carcinomamicroenvironment. It is hoped that the study can do a modest help toimprove of tumor vaccines, choose the suitable people for immunotherapyand estimate the prognosis.Objective: To analyse the various functional dendritic cells in prostatecarcinoma microenvironment. To investigate the relationship betweendendritic cells in prostate carcinoma microenvironment and various typesblood cells and its clinical prognostic evaluation.Methods: S-100+, CD83+and CD208+cells were determined byimmunohistochemical stain (MaxVision method) and collagen proteinswere determined by Masson’s Trichrome stain in16cases of benignprostatic hyperplasia and42cases of prostate carcinoma tissue. The amountof positive cells and collagen proteins were analysed by image processingsoftware.these positive cells, collagen proteins, blood cells and Gleasonsore were respectively dealt with the correlation analysis.Result: S-100, CD83positive cells and collagen proteins in benignprostatic hyperplasia tissue and prostate cancer tissue were statisticallysignificant difference (P <0.05). There was less S-100, CD83positive cellsand collagen proteins in prostate cancer tissue, CD208cells in benignprostatic hyperplasia tissue and prostate cancer tissue were not statisticallysignificant difference (P>0.05). There was a significant negative correlationbetween the cell index of S-100+DCs and Gleason score in prostate cancer (r=-0.533, P<0.01). Platelet count was significantly different betweengroup of benign prostatic hyperplasia and group of prostate cancer (P <0.05). There was more Platelets in prostate carcinoma tissue. High countsof mononuclear cells was possible a risk factor of prostate cancer (P <0.05). There was no significant difference between positive cells marked bydifferent antibodies and blood cells (P＞0.05).Conclusion: The quantity of S-100,CD83positive cells in the group ofbenign hyperplasia of prostate was significant more than the group ofprostate carcinoma, but there was no significant difference between thegroup of prostate carcinoma with bone metastases and the group of prostatecarcinoma without metastases. Platelet count was significantly differentbetween group of benign prostatic hyperplasia and group of prostate cancer(P=0.047). Platelet count showed normal distribution in group of benignprostatic hyperplasia and skewed distribution in group of prostatecarcinoma.Various dendritic cells count in prostate tissue and various typesblood cells count was uncorrelated. In the group of prostate carcinoma allkinds of dendritic cells count and collagen protein decreased and Plateletscount increased. S-100positive cells count was correlated with Gleasonscore in the prostate carcinoma.