The Roles Of Atorvastatin On The Expression Of Heparan Sulfates And Syndecans-1in Rats Myocardial Ischemia Reperfusion Injury

Objective: To observe the expression of heparan sulfates (HSPG) andSyndecans-1(SDC-1) in rats myocardial ischemia reperfusion injury, andevaluated the effects of atorvastatin.Methods:Forty healthy clean grade male Sprague Dawley（SD）ratswere randomly divided into four groups. There are10rats in every group,then myocardial ischemia reperfusion injury (MIRI) rat model wasestablished. The sham operation group, without coronary artery ligation. Theischemia reperfusion group (IR group), with the left anteriordescending coronary arteries (LAD) was ligated for30minutes and thenperforming reperfusion for120minutes. The atorvastatin group (atorvastatin20mg/kg/d dissolved with physiological saline into1ml) was givenatorvastatin by gastric irrigation for three days. The physiological salinegroup was given physiological saline1ml by gastric irrigation for three days.After-operation, the morphological changes of myocardium were observedby HE staining, the expression of HSPG and SDC-1in LAD and serumwere detected by immunohistochemistry、 reverse transcription polymerase chain reaction (RT-PCR) and enzymelinked immunosorbent assay (ELISA).Results:1. morphological changes under light microscopeMorphological changs of myocardial tissues was observed by using ofmicroscope after being stained by HE. Morphological character is almostnormal in group sham operation and group physiological saline, myocardialfibers in order, horizontal grain clarity, no inflammatory cells infiltration.Ischemia reperfusion myocardial cell nuclear enrichment microscope,nuclear shrinking, a large number of myocardial degeneration, focal necrosiscan be seen, myocardial fiber in disorders and with edema, a large number ofinflammatory cells infiltration, myocardial cell hardly damaged thanatorvastatin group and sham group. Atorvastatin group less degeneration ofmyocardial cells, we can see a small number of bleed focal necrosis,myocardial fibers with a relatively neat, a small amount of infiltration ofinflammatory cells, fibrous tissue lesions less than ischemia-reperfusiongroups.2. Light microscopy of exemplary LADs after immunohistochemicalstaining of HSPG and SDC-1Compared with sham operation group, in IR group, the content ofHSPG and SDC-1in LADs both significantly decreased (P＜0.05), butthere was no significant difference in physiological saline group(P＞0.05). Compared with IR group, in atorvastatin group, the contents of HSPG andSDC-1both significantly increased in LADs(P＜0.05).3. mRNA of HSPG and SDC-1in LADs detected by RT-PCRCompared with sham operation group, in IR group, the mRNA ofHSPG and SDC-1in LADs both significantly decreased (P＜0.05), butthere was no significant difference in physiological saline group(P＞0.05).Compared with IR group, in atorvastatin group, the mRNA of HSPG andSDC-1both significantly increased in LADs(P＜0.05).4. contents of HSPG and SDC-1in serum detected by ELISACompared with sham operation group, in IR group, the content ofHSPG and SDC-1both significantly increased in serum(P＜0.05), but therewas no significant difference in physiological saline group(P＞0.05).Compared with IR group, in atorvastatin group, the contents of HSPG andSDC-1both significantly decreased in serum(P＜0.05).Conclusions:1.HSPG and SDC-1could be injured by myocardial ischemiareperfusion.2.atorvastatin could increased myocardial protective effects in rats byreducing shedding of the endothelial glycocalyx.