Effects Of PEGFPN1-Dnegfr On Chemosensitivity To Oxaliplatin Of Gastric Carcinoma Cells

Objectives: To investigate the effects of pEGFPN1-dnEGFR onChemosensitivity of human gastric carcinoma cell lines SGC-7901andNCI-N87to oxaliplatin, and to elucidate the potential mechanism.Methods: Oxaliplatin in different concentrations were added to eachgroup, the dose-effect response was examined by MTT assay. The mRNAexpression of Caspase-3and Cyclin D1were detected by reversetranscription PCR (RT-PCR) in each group at24h after incubated withoxaliplatin. Protein expression of Caspase-3and Cyclin D1were detectedby Western blot.Results: pEGFPN1-dnEGFR could increase the sensitivity tooxaliplatin in both two human gastric carcinoma cell lines. The value ofinhibition (VI) of oxaliplatin in groups added with pEGFPN1-dnEGFR andoxaliplatin were significantly higher than those in other groups (P<0.05).RT-PCR results showed that mRNA expression of Cyclin D1was decreasedin cells tranfected with pEGFPN1-dnEGFR (P<0.05). In contrast,Caspase-3mRNA was increased in cells incubated with oxaliplatin for24h. Western blot results showed that tranfection with pEGFPN1-dnEGFR leadto a decrease of Cyclin D1protein expression, and an increase of Caspase-3protein expression after48h incubated with oxaliplatin (P<0.05).Conclusion: pEGFPN1-dnEGFR can enhance the chemosensitivity tooxaliplatin in human gastric carcinoma cells, which may be associated withCaspase-3and Cyclin D1.