The Effect And Significance Of Pmma In Primary Cultured Spinal Metastases Cell

BACKGROUND:Percutaneous vertebroplasty (PVP) is firstly reported in clinical application in1987and it has been widely used and achieved good results in the treatment of senile osteoporotic vertebral compression fracture and vertebral tumor. However, some issues still need further study.OBJECTIVE:To evaluate the effects of PMMA on the apoptosis and cell cycles of the primary cultured spinal metastases cells, to investigate the possible mechanisms of PMMA acting on spinal metastases in vitro and to provide experimental basis for clinical treatment of spinal metastasesMETHODS:Primary cultured spinal metastases cells were treated with PMMA of different stages for24h. Three groups were designed in this experiment:control, drawing-off stage PMMA group, cool stage PMMA group. The apoptosis of the spinal metastases cells and its relationship with cell cycles were determined by Annexin V-FITC/PI assay and flow cytometry. The expression of caspase-3、Caspase-8、 Caspase-9、Bax and Bcl-2,mRNA were observed by SYBR green I fluorescence quantitative PCR method. RESULTS:The cell apoptosis rates of the control, drawing-off stage PMMA group, cool stage PMMA group were (3.80±1.09)%、(43.46±8.55)%、(28.77±9.39)%, respectively, with the most significant increase in the combination group (P<0.05). Both groups of PMMA remarkably reduced the number of cells in the G0/G1phase, and increased that in the S phase (P<0.05). Drawing-off stage PMMA group can markedly increased the expression of caspase-3and Bax of primary cultured spinal metastases cells after treatment for24h, the expression of Bcl-2was significantly falled,but the expression of Caspase-8、Caspase-9do not have obvious change.CONCLUSION:PMMA can significantly induce their apoptosis and arrest their cell cycles in theS phase in vitro, as well as, with even more significant effect when used in drawing-off stage PMMA group than applied in cool stage.