In Situ Visual Imaging Of U14Head And Neck Squamous Cell Carcinoma Xenograft Tumors By Near-Infrared Quantum Dots-Monoclonal Antibody Fluorescent Probes

Objective: To study the in situ visual imaging of near-infrared quantumdots(NIRF-QDs) epidermal growth factor receptor monoclonalantibody(EGFR mAb) fluorescent probes on the U14head and necksquamous cell carcinoma xenograft tumors.Methods: Epidermal growth factor receptor monoclonal antibodieswere coupled to near-infrared fluorescent quantum dots (QD800) to preparenear-infrared fluorescent quantum dots-EGFR monoclonal antibodiesprobes (QD800-EGFR mAb). The squamous cell carcinoma U14with highexpression of EGFR and QD800-EGFR mAb were cocultured to observe thecombination in vitro;Visual in vivo imaging of U14head and neck squamouscell carcinoma xenograft tumors was done after intravenous injection ofQD800-EGFR mAb.Results: EGFR mAb successfully coupled to biological functions of theQD800,and it prepared to specific targeting fluorescent probesQD800-EGFR mAb;Experiments in vitro showed that QD800-EGFR mAbcould bind specifically to U14cells. In vivo imaging showed that clear visual in situ fluorescence imaging of head and neck squamous cell carcinomacould be achieved after intravenous injection of QD800-EGFR mAb, and thefluorescence imaging lasted up to24h during which the most complete andhighest signal to noise ratio of tumor in vivo imaging was in1～6h.Conclusion: EGFR target based near near-infrared quantum dotstechnique has unique advantages and prospects for the occurrence anddevelopment study, visual in situ imaging and individual treatment of headand neck squamous cell carcinoma.