In Situ Visual Imaging And The In Vivo Distribution Of Oral Squamous Cell Carcinoma In Mice By Near-infrared Quantum Dots Egfr Monoclonal Antibody Fluorescent Probes

Objective: To investigate the in situ visualization imaging and the invivo distribution of the near-infrared quantum dot epidermal growth factorreceptor(EGFR) monoclonal antibody fluorescent probes in oral squamouscell carcinoma (OSCC).Methods: Quantum dots with an emission wavelength of800nm(QD800) was conjugated with monoclonal antibodies against EGFR toproduce QD800-EGFR mAb. QD800-EGFR mAb was co-cultured withBcaCD885squamous cancer cell line with high expression of EGFR for30min and observed with laser passing confocal microscopy. The humanbuccal squamous cell carcinoma BcaCD885cells were injected into nudemice cheek subcutaneous to establish the nude mouse model.QD800-EGFR mAb was injected into OSCC animal model through the tailvein and the in situ visualization imaging and the in vivo distribution ofQD800-EGFR mAb was analyzed at different time points. Results: QD800-EGFR mAb could specifically bind to EGFR onBcaCD885cell membrane in vitro. BcaCD885squamous cell carcinoma inthe oral can be clearly visualization imaging after intravenous injection ofQD800-EGFR Ab probes, these fluorescence signals lasted for24hours.The most complete tumor images with maximal signal-to-noise ratio wereobserved from30min to6h after injection of the probe. In vivo tissuedistribution studies demonstrated that QD800aggregated most in liver.QD800aggregated decreased with time going by in tumors, and QD800didn’t aggregated in heart, brain, intestine, lung and stomach.Conclusion: The QD800mAb probe can produce clear visualimages in oral cancer. It has promising prospects in visualization in vivoimaging and individual treatment of oral cancer. However, the studies ofdecrease the aggregation of QD800-EGFR mAb probe in liver areimportant directions for future investigations.