Investigation Of The Inlfuence Of Deferoxamine On The Survival Of A Random Pattern Skin Flap Of The Diabetic Mouse

Objective: Deferoxamine was injected into the flap of the mouse’s back. We obse-rved the area of random flap survival and discussed the flap survival mechanism for theincreased skin graft success rate. We wish this paper could provide a theoretical basisfor clinical treatment.Methods: We selected30black adult C57/BL6mice which were induced diabetesmellitus with streptozotocin.They were randomly divided into treatment groups DFO,PBS control group, BLANK control group, ten in each group. Random flap of1×3cmwere created on its back. DFO with the phosphate buffer solution, then we injected thisDFO solution into the flap. DFO was administered once after surgery, after the deliverytime once a day for3days. DFO group: flap injected100mg/kg0.3ml; PBS group: inj-ection of sterile flap PBS0.3ml; BLANK control group: the flap is not injected.7daysafter flap survival was measured in length, the calculation of flap survival; observed inHE sections flap layers structure and inflammatory cell infiltration; CD31+cells by im-munofluorescence staining to calculate microvascular density; laser Doppler and imageanalysis to compare the blood flow of flap; Western-blot to compare the HIF-1α andVEGF expression in the flap’s distal.Results:1.DFO group of flap survival area (88.44%±6.56) was significantlygreater than the PBS media group (61.64%±7.63)(P <0.05).2.HE: DFO groupsubcutaneous tissue edema, vascular dilation, vascular hyperplasia and massiveneutrophil infiltration, the basic integrity of the meat membrane; PBS group:Subcutaneous tissue was significantly reduced, diffuse subcutaneous infiltration ofinflammatory cells, A small amount of meat membrane be remained and theproliferation of vascular; BLANK group: Diffuse subcutaneous infiltration ofinflammatory cells and no angiogenesis.3. Capillary density: DFO group8.33±1.25/mm~2; PBS group3.33±0.47/mm~2; BLANK group3.67±0.46/mm~2.4. LaserDoppler results: DFO treatment group, the average blood flow (565.03±13.15) and the PBS group (417.53±14.89) were significantly different (P <0.05).5.Western-blotdetection of HIF-1α and VEGF expression in the DFO treated group29.54±0.68; PBStreated group7.29±0.51; BLANK no treatment group7.79±0.37.Conclusion: DFO diabetic C57/BL6mice can promote the survival of random flapin the back. The rate of skin flap survival, the blood flow of skin flap, microvesseldensity, HIF-1α and VEGF show the differences. DFO, in mechanism, may increaseHIF-1α, VEGF, and thus lead to capillary proliferation, increased blood blow.