Changes Of Adrenomedullin And Its Relationship With Brain Tissue Contentration Of Superoxide Dismutase And Water In Rats With Cerebral Hemorrhage

Part1: Establishment of moderate to severe cerebral hemorrhage induced model by injection type Ⅳcollagenase with different doses into caudate putamen nuelei in ratsObjective:To establish a reliable and moderate to severe intracerebral hemorrhage model in rats by injection of type Ⅳ collagenase and heparin into caudate putamen nuclei.Methods:A total of66SD rats were randomly divided into Ⅳ collagenase0.4U+heparin4U injection group (A group,12), Ⅳ collagenase0.6U+heparin4U injection group (B group,42rats)and Ⅳ collagenase0.8U+heparin4U injection group (C group,12rats). The neurological behavior scores of rats were assessed at24hours、72hours after the injection with A and B groups and were assessed at4hours、12hours、24hours、48hours、72hours、5days and7days after injection with B group. The volume of hematoma and edema was detected by multimedia pathological imaging analysis system.Results:The scores of neurological behavior, volume of hematoma and edema among group A, group B and group C showed obvious difference (P<0.01).The group of A has less the volume of hematoma, the volume of edema and neurological deficits, the others not only have more the volume of hematoma and the volume of edema, but also have more serious neurological deficits. However, the mortality at72hours after injection was37%in group C, much higher than that in other groups.Conclusion:A reliable and moderate to severe intracerebral hemorrhage model in rats can be induced by injection of0.6U IV collagenase with4U heparin into caudate putamen nuclei. The model has obviously neurological deficit and a high persistence. This is a useful and reliable method to study intracerebral hemorrhage. Part2Changes of Adrenomedullin and Its Relationship with Brain Tissue Contentration of Superoxide Dismutase and water in Rats with Cerebral hemorrhage and ReperfusionObjective:To investigate the changes of plasma adrenomedullin and its relationship with brain tissue concentration of superoxide dismutase and water after basal nuclei cerebral hemorrhage and reperfusion in rats.Methods:Sixty SD rats were divided into normal group (group A), sham operation group (group B) and model group (group C). According to the time of the ICH formation,48rats of group C were divided into8groups:4hours、12hours、24hours、2days、3days、5days、7days and14days.Every group contains six rats. The plasma concentration of adrenomedullin in group C was determined by using enzyme linked immunosorbent assay, the brain tissue concentration of superoxide dismutase in group C was determined by using xanthine oxidase assay and the brain edema in group C was determined by using wet/dry weight assay and compared with those in group A and group B.Results:The plasma concentration of adrenomedullin、the brain edema and the neurological behavior scores in group C was significantly increased and the brain tissue vitality of superoxide dismutase in group C was significantly decreased in rats with cerebral hemorrhage and reperfusion at acute stage as compared with that in group A and B (P<0.01).But the group A compare with group B wasn’t significant difference (P>0.05). The level of adrenomedullin reached the peak at4hour after cerebral hemorrhage and obviously decreased at24hours. But the level of adrenomedullin reached the peak once again at3days after cerebral hemorrhage and gradually resolved in7-14days. The brain edema was obvious at24hour after cerebral hemorrhage, peaked at2-3days and gradually resolved normal in7-14days. The neurological behavior scores were obvious at4hours after cerebral hemorrhage, peaked at2-3days and gradually resolved normal in7-14days. The brain tissue vitality of superoxide dismutase was decreased obviously at12hours after cerebral hemorrhage, low at3days and gradually resolved normal in7-14days.Conclusion:The brain edema and the neurological behavior scores in group C were significantly increased and the brain tissue vitality of superoxide dismutase in group C was significantly decreased in rats with cerebral hemorrhage and reperfusion at acute stage. There are gradually resolved in7-14days. The level of adrenomedullin reached the peak at4hours after cerebral hemorrhage and obviously decreased at24hours. The exogenous ADM may lessen the brain edema, improve the neurological behavior and protect nerve cell, because it can broaden vasa sanguinea and improve cerebral blood flow.