The Study On The Relativity Between Plasma Visfatin, NO And Type2Diabetes And Diabetic Nephropathy

Objective: To detect the change of level of Visfatin, nitric oxide (NO) and relatedmetabolic parameters in type2diabetes and its complications of disbetic nephropathy.Evaluate the relationship between Visfatin,NO and related metabolic markers,andfurther to explore their roles in the development of type2diabetes mellitus and diabeticnephropathy.Methods:72patients with type2diabetes were selected as a case group (T2DM),which including40cases with complicated diabetic nephropathy（DN）, and32casesof no-complication group (Diabetes Mellitus, DM); choose28cases of the age and BMImatched healthy people as control group (NC).Plasma Visfatin levels were detected byEnzyme-Linked-Immunosorbent-Assay and Nitrate Reductase Assay were used tomeasure the level of plasma NO. In the meantime blood glucose, blood lipids, insulin,proinsulin, glucagon, body mass index, waist-hip ratio, high sensitivity CRP, and otherrelated indicators were also measured.Insulin resistance index of HOMA-IR was chosento evaluate insulin resistance. SPSS16.0software was used for medical statistics andcorrelation analysis.Results:1.The plasm Visfatin level of type2diabetic patients withoutcomplications(DM), diabetic nephropathy (DN) and the control group (NC) were47.46±3.94ng/ml,50.24±4.93ng/ml,27.22±4.39ng/ml,respectively.The levels of plasmVisfatin among the three groups were significantly different (P<0.05). Theconcentration of plasma Visfatin in DN was higher than DM group (P<0.05). Theconcentration of plasma Visfatin in DM group wsa higher than NC group (P <0.05);2.The plasm NO levels of type2diabetic patients without complications (DM), diabeticnephropathy (DN) and the control group (NC) plasma were52.05±6.14umol/L,44.05±6.43umol/L,61.01±4.76, umol/L,respectively. The levels of plasma NO amongthe three groups were of significant differenence (P <0.05). The concentration ofplasma NO in DN was lower than DM group (P <0.05), The concentration of plasmaNO in DM group was lower than NC group (P <0.05);3.Correlation analysis showed that plasma visfatin in type2diabetes was negativelycorrelated with NO concentration, however was positively correlated with WC, WHR,TG, waist to height ratio, FINS, HOMA-IR.We further conduct multivariate regression analysis using Visfatin as a dependent variable, and age as independent variable, NO,waist-hip ratio, HOMA-IR were entered into the regression equation. It was suggestedthat type2diabetes, plasma NO concentration was negatively correlated with visfatin,HOMA-IR, HOMA-β, FINS, hsCRP, WC, WHR, TG(P <0.05or P <0.01), but waspositively correlated with HDL-C. NO was selected as the dependent variable, and ageas independent variable in multivariate regression analysis, Visfatin, HOMA-IR,C-reactive protein were entered into the regression equation.Conclusion:1. Plasma Visfatin level in DN group is significantly higher than the DMgroup, Plasma Visfatin levels of DM group is significantly higher than control group,suggesting that Visfatin is related with the onset and development of type2diabetes anddiabetic nephropathy;2.Plasma NO level in DN group is significantly lower than the DM group, Plasma NOlevels of DM group is significantly lower than control group, suggesting that NO isrelated with the onset and development of type2diabetes and diabetic nephropathy;3.Visfatin and NO can be used as observed indexes of diabetic nephropathy in type2diabetes mellitus complication;4.Visfatin, NO and insulin resistance may be related to type2diabetes and diabeticnephropathy in the pathogenesis of insulin resistance, vascular endothelial celldysfunction are closely related.